Analysis of Extracellular Vesicle MicroRNA Profiles Reveals Distinct Blood and Lymphatic Endothelial Cell Origins

Overview

Journal J Extracell Biol

Date 2024 Jun 28

PMID 38938681

Authors

Marianne Pultar

Johannes Oesterreicher

Jaana Hartmann

Moritz Weigl

Andreas Diendorfer

Katharina Schimek

Barbara Schadl

Thomas Heuser

Marlene Brandstetter

Johannes Grillari

Peter Sykacek

Matthias Hackl

Wolfgang Holnthoner

Affiliations

Soon will be listed here.

Abstract

Extracellular vesicles (EVs) are crucial mediators of cell-to-cell communication in physiological and pathological conditions. Specifically, EVs released from the vasculature into blood were found to be quantitatively and qualitatively different in diseases compared to healthy states. However, our understanding of EVs derived from the lymphatic system is still scarce. In this study, we compared the mRNA and microRNA (miRNA) expression in blood vascular (BEC) and lymphatic (LEC) endothelial cells. After characterization of the EVs by fluorescence-triggered flow cytometry, nanoparticle tracking analysis and cryo-transmission electron microscopy (cryo-TEM) we utilized small RNA-sequencing to characterize miRNA signatures in the EVs and identify cell-type specific miRNAs in BEC and LEC. We found miRNAs specifically enriched in BEC and LEC on the cellular as well as the extracellular vesicle level. Our data provide a solid basis for further functional in vitro and in vivo studies addressing the role of EVs in the blood and lymphatic vasculature.

Citing Articles

Analysis of extracellular vesicle microRNA profiles reveals distinct blood and lymphatic endothelial cell origins.

Pultar M, Oesterreicher J, Hartmann J, Weigl M, Diendorfer A, Schimek K J Extracell Biol. 2024; 3(1):e134.

PMID: 38938681 PMC: 11080916. DOI: 10.1002/jex2.134.

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