LABS: Linear Amplification-based Bisulfite Sequencing for Ultrasensitive Cancer Detection from Cell-free DNA

Overview

Journal Genome Biol

Specialties Biology
Genetics

Date 2024 Jun 14

PMID 38877540

Authors

Xiao-Long Cui

Ji Nie

Houxiang Zhu

Krissana Kowitwanich

Alana V Beadell

Diana C West-Szymanski

Zhou Zhang

Urszula Dougherty

Akushika Kwesi

Zifeng Deng

Yan Li

Danqing Meng

Kevin Roggin

Teresa Barry

Ryan Owyang

Ben Fefferman

Chang Zeng

Lu Gao

Carolyn W T Zhao

Yuri Malina

Jiangbo Wei

Melanie Weigert

Wenjun Kang

Ajay Goel

Brian C-H Chiu

Marc Bissonnette

Wei Zhang

Mengjie Chen

Chuan He

Affiliations

Soon will be listed here.

Abstract

Methylation-based liquid biopsies show promises in detecting cancer using circulating cell-free DNA; however, current limitations impede clinical application. Most assays necessitate substantial DNA inputs, posing challenges. Additionally, underrepresented tumor DNA fragments may go undetected during exponential amplification steps of traditional sequencing methods. Here, we report linear amplification-based bisulfite sequencing (LABS), enabling linear amplification of bisulfite-treated DNA fragments in a genome-wide, unbiased fashion, detecting cancer abnormalities with sub-nanogram inputs. Applying LABS to 100 patient samples revealed cancer-specific patterns, copy number alterations, and enhanced cancer detection accuracy by identifying tissue-of-origin and immune cell composition.

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