Compositional Analysis of Complex Mixtures Using Automatic MicroED Data Collection

Overview

Journal Adv Sci (Weinh)

Specialty Biomedical Engineering

Date 2024 Apr 22

PMID 38647272

Authors

Johan Unge

Jieye Lin

Sara J Weaver

Ampon Sae Her

Tamir Gonen

Affiliations

Soon will be listed here.

Abstract

Quantitative analysis of complex mixtures, including compounds having similar chemical properties, is demonstrated using an automatic and high throughput approach to microcrystal electron diffraction (MicroED). Compositional analysis of organic and inorganic compounds can be accurately executed without the need of diffraction standards. Additionally, with sufficient statistics, small amounts of compounds in mixtures can be reliably detected. These compounds can be distinguished by their crystal structure properties prior to structure solution. In addition, if the crystals are of good quality, the crystal structures can be generated on the fly, providing a complete analysis of the sample. MicroED is an effective method for analyzing the structural properties of sub-micron crystals, which are frequently found in small-molecule powders. By developing and using an automatic and high throughput approach to MicroED, and with the use of SerialEM for data collection, data from thousands of crystals allow sufficient statistics to detect even small amounts of compounds reliably.

Citing Articles

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Reaching the potential of electron diffraction.

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Compositional Analysis of Complex Mixtures using Automatic MicroED Data Collection.

Unge J, Lin J, Weaver S, Her A, Gonen T Adv Sci (Weinh). 2024; 11(23):e2400081.

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Electron counting with direct electron detectors in MicroED.

Hattne J, Clabbers M, Martynowycz M, Gonen T Structure. 2023; 31(12):1504-1509.e1.

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