Single Cell Characterization of Blood and Expanded Regulatory T cells in Autoimmune Polyendocrine Syndrome Type 1

Overview

Journal iScience

Publisher Cell Press

Date 2024 Apr 18

PMID 38632993

Authors

Thea Sjogren

Shahinul Islam

Igor Filippov

Adrianna Jebrzycka

Andre Sulen

Lars E Breivik

Alexander Hellesen

Anders P Jorgensen

Kari Lima

Liina Tserel

Kai Kisand

Part Peterson

Annamari Ranki

Eystein S Husebye

Bergithe E Oftedal

Anette S B Wolff

Affiliations

Soon will be listed here.

Abstract

Immune tolerance fails in autoimmune polyendocrine syndrome type 1 (APS-1) because of mutations. We have used single cell transcriptomics to characterize regulatory T cells (Tregs) sorted directly from blood and from expanded Tregs in APS-1 patients compared to healthy controls. We revealed only CD52 and LTB (down) and TXNIP (up) as consistently differentially expressed genes in the datasets. There were furthermore no large differences of the TCR-repertoire of expanded Tregs between the cohorts, but unique patients showed a more restricted use of specific clonotypes. We also found that expanded Tregs from APS-1 patients had similar suppressive capacity as controls in co-culture assays, despite expanding faster and having more exhausted cells. Our results suggest that APS-1 patients do not have intrinsic defects in their Treg functionality, and that their Tregs can be expanded for potential therapeutic applications.

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