Prophage Terminase with TRNase Activity Sensitizes to Oxidative Stress

Overview

Journal Science

Specialty Science

Date 2024 Apr 4

PMID 38574144

Authors

Siva Uppalapati

Sashi Kant

Lin Liu

Ju-Sim Kim

David Orlicky

Michael McClelland

Andres Vazquez-Torres

Affiliations

Soon will be listed here.

Abstract

Phage viruses shape the evolution and virulence of their bacterial hosts. The genome encodes several stress-inducible prophages. The Gifsy-1 prophage terminase protein, whose canonical function is to process phage DNA for packaging in the virus head, unexpectedly acts as a transfer ribonuclease (tRNase) under oxidative stress, cleaving the anticodon loop of tRNA. The ensuing RNA fragmentation compromises bacterial translation, intracellular survival, and recovery from oxidative stress in the vertebrate host. adapts to this transfer RNA (tRNA) fragmentation by transcribing the RNA repair Rtc system. The counterintuitive translational arrest provided by tRNA cleavage may subvert prophage mobilization and give the host an opportunity for repair as a way of maintaining bacterial genome integrity and ultimately survival in animals.

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