Evaluation of Oxacillin Tolerance in Staphylococcus Aureus by a Novel Method

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 1985 Sep 1

PMID 3852657

Citations 14

Authors

B F WOOLFREY

R T Lally

M N Ederer

Affiliations

Soon will be listed here.

Abstract

A novel agar dilution plate-count procedure for the quantitative measurement of bacterial inhibition and killing is described. For Staphylococcus aureus versus oxacillin, by the agar dilution plate-count procedure it was found that only 1 of 20 clinical isolates and 1 of 7 allegedly tolerant reference isolates met the conventional definition of tolerance. By using inocula of 10(5) CFU per plate, most isolates were demonstrated to have subpopulations of cells which, although inhibited, persisted for 24 h in concentrations significantly above their MICs. The persister percentages at 24 h appeared to be strain dependent, and all persisters exhibited the paradoxical effect. For each isolate, the persister percentage markedly decreased after action by oxacillin for 48 h, and the paradoxical effect was greatly diminished. Our findings suggest that tolerance is an artificial and arbitrary concept that does not adequately characterize the inhibition and killing dynamics associated with the persister phenomenon.

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Influence of technical factor variations during inoculum preparation on the agar dilution plate-count method for quantitation of Staphylococcus aureus oxacillin persisters.

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Oxacillin killing curve patterns of Staphylococcus aureus isolates by agar dilution plate count method.

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References

Pearson R, Steigbigel R, Davis H, Chapman S . Method of reliable determination of minimal lethal antibiotic concentrations. Antimicrob Agents Chemother. 1980; 18(5):699-708. PMC: 284079. DOI: 10.1128/AAC.18.5.699. View

Venglarcik 3rd J, Blair L, Dunkle L . pH-dependent oxacillin tolerance of Staphylococcus aureus. Antimicrob Agents Chemother. 1983; 23(2):232-5. PMC: 186027. DOI: 10.1128/AAC.23.2.232. View

Sabath L, Wheeler N, Laverdiere M, BLAZEVIC D, Wilkinson B . A new type of penicillin resistance of Staphylococcus aureus. Lancet. 1977; 1(8009):443-7. DOI: 10.1016/s0140-6736(77)91941-9. View

Zinner S, Husson M, Klastersky J . Effect of mixing on rifampin bactericidal activity against staphylococci. Antimicrob Agents Chemother. 1981; 20(2):267-9. PMC: 181678. DOI: 10.1128/AAC.20.2.267. View

Eagle H . Further observations on the zone phenomenon in the bactericidal action of penicillin. J Bacteriol. 1951; 62(5):663-8. PMC: 386183. DOI: 10.1128/jb.62.5.663-668.1951. View

Ishida K, Guze P, KALMANSON G, Albrandt K, Guze L . Variables in demonstrating methicillin tolerance in Staphylococcus aureus strains. Antimicrob Agents Chemother. 1982; 21(4):688-90. PMC: 181972. DOI: 10.1128/AAC.21.4.688. View

Horne D, Tomasz A . pH-dependent penicillin tolerance of group B streptococci. Antimicrob Agents Chemother. 1981; 20(1):128-35. PMC: 181644. DOI: 10.1128/AAC.20.1.128. View

GUNNISON J, FRAHER M, JAWETZ E . PERSISTENCE OF STAPHYLOCOCCUS AUREUS IN PENICILLIN IN VITRO. J Gen Microbiol. 1964; 35:335-49. DOI: 10.1099/00221287-35-2-335. View

Kim K, Anthony B . Penicillin tolerance in group B streptococci isolated from infected neonates. J Infect Dis. 1981; 144(5):411-9. DOI: 10.1093/infdis/144.5.411. View

10.

Peterson L, Gerding D, Hall W, Schierl E . Medium-dependent variation in bactericidal activity of antibiotics against susceptible Staphylococcus aureus. Antimicrob Agents Chemother. 1978; 13(4):665-8. PMC: 352307. DOI: 10.1128/AAC.13.4.665. View

11.

Tomasz A, Albino A, Zanati E . Multiple antibiotic resistance in a bacterium with suppressed autolytic system. Nature. 1970; 227(5254):138-40. DOI: 10.1038/227138a0. View

12.

McDermott W . Microbial persistence. Yale J Biol Med. 1958; 30(4):257-91. PMC: 2603844. View

13.

Mayhall C, Medoff G, Marr J . Variation in the susceptibility of strains of Staphylococcus aureus to oxacillin, cephalothin, and gentamicin. Antimicrob Agents Chemother. 1976; 10(4):707-12. PMC: 429818. DOI: 10.1128/AAC.10.4.707. View

14.

Park J, Edwards J, WISE Jr E . In vivo studies on the uptake and binding of beta-lactam antibiotics in relation to inhibition of wall synthesis and cell death. Ann N Y Acad Sci. 1974; 235(0):300-9. DOI: 10.1111/j.1749-6632.1974.tb43273.x. View

15.

Goessens W, Fontijne P, Michel M . Factors influencing detection of tolerance in Staphylococcus aureus. Antimicrob Agents Chemother. 1982; 22(3):364-8. PMC: 183749. DOI: 10.1128/AAC.22.3.364. View

16.

Shah P . Paradoxical effect of antibiotics. I. The 'Eagle effect'. J Antimicrob Chemother. 1982; 10(4):259-60. DOI: 10.1093/jac/10.4.259. View

17.

Goessens W, Fontijne P, Michel M . Responses of tolerant and nontolerant Staphylococcus aureus strains to methicillin treatment in an experimental infection in mice. Antimicrob Agents Chemother. 1984; 26(6):829-32. PMC: 180033. DOI: 10.1128/AAC.26.6.829. View

18.

Greenwood D . Mucopeptide hydrolases and bacterial "persisters". Lancet. 1972; 2(7775):465-6. DOI: 10.1016/s0140-6736(72)91858-2. View

19.

Goessens W, Fontijne P, van Raffe M, Michel M . Tolerance percentage as a criterion for the detection of tolerant Staphylococcus aureus strains. Antimicrob Agents Chemother. 1984; 25(5):575-8. PMC: 185589. DOI: 10.1128/AAC.25.5.575. View

20.

Masuda G, Tomioka S, Uchida H, Hasegawa M . Bacteriostatic and bactericidal activities of selected beta-lactam antibiotics studied on agar plates. Antimicrob Agents Chemother. 1977; 11(3):376-82. PMC: 351992. DOI: 10.1128/AAC.11.3.376. View