Influence of Technical Factor Variations on Serum Inhibition and Bactericidal Titers

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1986 Jun 1

PMID 3486883

Citations 3

Authors

B F WOOLFREY

R T Lally

K R Tait

Affiliations

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Abstract

The influence of technical factor variations on serum bactericidal and serum inhibitory titers was studied by using Staphylococcus aureus clinical isolates versus oxacillin-spiked human serum. Parallel tests, both with and without the use of beta-lactamase in count plates to inactivate oxacillin carryover, were performed with a conventional macrodilution approach, a carefully controlled macrodilution procedure, and a standard microdilution method. Careful control of technical factor variations diminished the incidence of low serum bactericidal titers and decreased the dispersion of results, a finding corollary to the known influence of technical factor variations on the measurement of MBCs. The incorporation of beta-lactamase into count plates resulted in a shift of serum bactericidal titers to lower values. The microdilution method appeared to be least influenced by technical variations and, with the addition of beta-lactamase to count plates, provided the best results.

Citing Articles

Oxacillin, cephalothin, and vancomycin tube macrodilution MBC result reproducibility and equivalence to MIC results for methicillin-susceptible and reputedly tolerant Staphylococcus aureus isolates.

Pelletier Jr L, Baker C Antimicrob Agents Chemother. 1988; 32(3):374-7.

PMID: 3364955 PMC: 172179. DOI: 10.1128/AAC.32.3.374.

Serum bactericidal test.

Stratton C Clin Microbiol Rev. 1988; 1(1):19-26.

PMID: 3060242 PMC: 358026. DOI: 10.1128/CMR.1.1.19.

Tests for bactericidal effects of antimicrobial agents: technical performance and clinical relevance.

Peterson L, Shanholtzer C Clin Microbiol Rev. 1992; 5(4):420-32.

PMID: 1423219 PMC: 358258. DOI: 10.1128/CMR.5.4.420.

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