In Vivo DNA Assembly Using the PEDA Method

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2024 Mar 12

PMID 38468102

Authors

Tianyuan Su

Qingxiao Pang

Qingsheng Qi

Affiliations

Soon will be listed here.

Abstract

Simple and efficient DNA assembly methods have been widely used in synthetic biology. Here, we provide the protocol for the recently developed PEDA (phage enzyme-assisted in vivo DNA assembly) method for direct in vivo assembly of individual DNA parts in multiple microorganisms, such as Escherichia coli, Ralstonia eutropha, Pseudomonas putida, Lactobacillus plantarum, and Yarrowia lipolytica. PEDA allows in vivo assembly of DNA fragments with homologous sequences as short as 5 bp, and the efficiency is comparable to the prevailing in vitro DNA assembly, which will broadly boost the rapid progress of synthetic biology.

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