Recent Advances in DNA Assembly Technologies

Overview

Journal FEMS Yeast Res

Publisher Oxford University Press

Specialty Microbiology

Date 2014 Jun 7

PMID 24903193

Citations 53

Authors

Ran Chao

Yongbo Yuan

Huimin Zhao

Affiliations

Soon will be listed here.

Abstract

DNA assembly is one of the most important foundational technologies for synthetic biology and metabolic engineering. Since the development of the restriction digestion and ligation method in the early 1970s, a significant amount of effort has been devoted to developing better DNA assembly methods with higher efficiency, fidelity, and modularity, as well as simpler and faster protocols. This review will not only summarize the key DNA assembly methods and their recent applications, but also highlight the innovations in assembly schemes and the challenges in automating the DNA assembly methods.

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