Spectral Flow Cytometry Methods and Pipelines for Comprehensive Immunoprofiling of Human Peripheral Blood and Bone Marrow

Overview

Journal Cancer Res Commun

Specialty Oncology

Date 2024 Mar 11

PMID 38466569

Authors

Milos Spasic

Esther R Ogayo

Adrienne M Parsons

Elizabeth A Mittendorf

Peter van Galen

Sandra S McAllister

Affiliations

Soon will be listed here.

Abstract

Significance: This study introduces optimized methods and analysis algorithms that enhance capabilities in comprehensive immunophenotyping of human blood and bone marrow using spectral flow cytometry. This approach facilitates detection of rare cell types, enables measurement of cell variations across donors, and provides proof-of-concept in identifying known hematologic malignancies. By unlocking complexities of hematopoietic and immune landscapes at the single-cell level, this advancement holds potential for understanding disease states and therapeutic responses.

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