Spectral Flow Cytometry

Overview

Journal Curr Protoc Cytom

Publisher Wiley

Specialty Cell Biology

Date 2013 Jan 8

PMID 23292705

Citations 57

Authors

John P Nolan

Danilo Condello

Affiliations

Soon will be listed here.

Abstract

Interest in measuring the complete fluorescence spectra of individual cells in flow can be traced to the earliest days of flow cytometry. Recent advances in detectors, optics, and computation have made it possible to make full spectral measurements in the sub-millisecond time frame in which flow cytometry measurements typically occur. This opens up new possibilities for applying spectroscopy to the analysis of individual cells. This unit reviews historical and contemporary approaches to spectral flow cytometry, as well as instrument design, calibration, and data analysis for spectral flow cytometry applications.

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