Sequencing Analysis and Efficient Biodiesel Production by Lipase from Pseudomonas Aeruginosa

Overview

Journal Mol Biol Rep

Specialty Molecular Biology

Date 2024 Feb 23

PMID 38393680

Authors

Israa M S Al-Kadmy

Sarah Naji Aziz

Nadheema Hammood Hussein

Samah N El-Shafeiy

Israa Hussein Hamzah

Ahmed Suhail

Eman Alhomaidi

Abdelazeem M Algammal

Gaber El-Saber Batiha

Hala M ElBadre

Helal F Hetta

Affiliations

Soon will be listed here.

Abstract

Background: Recently, lipase processing for biodiesel production has shown a global increase as it is considered a potential alternative clean-fuel source. The current study's objective is to investigate of lipolytic activity of lipase produced from different strains of Pseudomonas aeruginosa (P. aeruginosa) in biodiesel production using edible plant oils. The goal is to develop an efficient and cost-effective method for producing inexpensive and environmentally friendly biodiesel.

Methods And Results: Four P. aeruginosa isolates were obtained from different environmental sources (soil), phenotypically identified, and it was confirmed by the PCR detection of the 16SrRNA gene. The isolated P. aeruginosa strains were screened for lipase production, and the recovered lipase was purified. Besides, the lipase (lip) gene was detected by PCR, and the purified PCR products were sequenced and analyzed. The production of biofuel was conducted using gas chromatography among tested oils. It was found that castor oil was the best one that enhances lipase production in-vitro.

Citing Articles

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Hetta H, Ramadan Y, Rashed Z, Alharbi A, Alsharef S, Alkindy T Molecules. 2024; 29(15).

PMID: 39124871 PMC: 11313800. DOI: 10.3390/molecules29153466.

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