Research Progress in Molecular Biology Related Quantitative Methods of MicroRNA

Overview

Journal Am J Transl Res

Specialty General Medicine

Date 2020 Aug 11

PMID 32774694

Citations 13

Authors

Kangsheng Liu

Hua Tong

Taiping Li

Xiangdong Wang

Yajun Chen

Affiliations

Soon will be listed here.

Abstract

MicroRNAs (miRNAs) are small RNAs of 18-25 nucleotides in length that are widely distributed in eukaryotes and are produced by DNA transcription. As regulators of post-transcriptional gene expression, it plays an important role in the physiological processes of cells. As some miRNAs in the body are abnormally expressed at different and earlier stages of diseases, this phenomenon suggests that accurate, sensitive and specifical detection of them can be helpful for early and differential diagnosis. To expound the technological progress of miRNA detection, we reviewed all the related articles in PubMed database published before May 6, 2019, with the following keywords: "miRNA", "real-time fluorescent quantitative PCR", "electrochemical detection", "next-generation sequencing", "digital PCR technology". Original articles and reviews on the topics were selected. The present methods established for quantitative detection of miRNAs mainly relies on various probe design and labeling techniques, and the improvement of the sensitivity and specificity of detection is often through combination of microarray chips, real-time fluorescent quantitative PCR, high-throughput sequencing and other techniques. This paper combines the existing microRNA detection methods to provide a reference for researchers to choose the best detection method.

Citing Articles

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Bta-miR-365-3p-targeted FK506-binding protein 5 participates in the AMPK/mTOR signaling pathway in the regulation of preadipocyte differentiation in cattle.

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Sequencing analysis and efficient biodiesel production by lipase from Pseudomonas aeruginosa.

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Ultrafast Microfluidic PCR Thermocycler for Nucleic Acid Amplification.

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