» Articles » PMID: 25573113

Molecular and Functional Diversity of Yeast and Fungal Lipases: Their Role in Biotechnology and Cellular Physiology

Overview
Journal Prog Lipid Res
Specialty Biochemistry
Date 2015 Jan 10
PMID 25573113
Citations 34
Authors
Affiliations
Soon will be listed here.
Abstract

Lipase catalyzes hydrolysis of fats in lipid water interphase and perform variety of biotransformation reactions under micro aqueous conditions. The major sources include microbial lipases; among these yeast and fungal lipases are of special interest because they can carry out various stereoselective reactions. These lipases are highly diverse and are categorized into three classes on the basis of oxyanion hole: GX, GGGX and Y. The detailed phylogenetic analysis showed that GX family is more diverse than GGGX and Y family. Sequence and structural comparisons revealed that lipases are conserved only in the signature sequence region. Their characteristic structural determinants viz. lid, binding pocket and oxyanion hole are hotspots for mutagenesis. Few examples are cited in this review to highlight the multidisciplinary approaches for designing novel enzyme variants with improved thermo stability and substrate specificity. In addition, we present a brief account on biotechnological applications of lipases. Lipases have also gained attention as virulence factors, therefore, we surveyed the role of lipases in yeast physiology related to colonization, adhesion, biofilm formation and pathogenesis. The new genomic era has opened numerous possibilities to genetically manipulate lipases for food, fuel and pharmaceuticals.

Citing Articles

Comprehensive Insights into Microbial Lipases: Unveiling Structural Dynamics, Catalytic Mechanism, and Versatile Applications.

Shah H, Zhang C, Khan S, Patil P, Li W, Xu Y Curr Microbiol. 2024; 81(11):394.

PMID: 39375258 DOI: 10.1007/s00284-024-03904-5.


Use of genomics & proteomics in studying lipase producing microorganisms & its application.

Majumder D, Dey A, Ray S, Bhattacharya D, Nag M, Lahiri D Food Chem (Oxf). 2024; 9:100218.

PMID: 39281291 PMC: 11402113. DOI: 10.1016/j.fochms.2024.100218.


Utilization of Fusarium Solani lipase for enrichment of polyunsaturated Omega-3 fatty acids.

de Carvalho Silva A, Lima F, Borges K, Wolff L, de Andrade M, Alves R Braz J Microbiol. 2024; 55(3):2211-2226.

PMID: 38874742 PMC: 11405586. DOI: 10.1007/s42770-024-01411-0.


Sequencing analysis and efficient biodiesel production by lipase from Pseudomonas aeruginosa.

Al-Kadmy I, Aziz S, Hussein N, El-Shafeiy S, Hamzah I, Suhail A Mol Biol Rep. 2024; 51(1):323.

PMID: 38393680 DOI: 10.1007/s11033-023-09156-z.


Uptake and Metabolic Conversion of Exogenous Phosphatidylcholines Depending on Their Acyl Chain Structure in .

Kotlova E, Senik S, Pozhvanov G, Prokopiev I, Boldyrev I, Manzhieva B Int J Mol Sci. 2024; 25(1).

PMID: 38203257 PMC: 10778594. DOI: 10.3390/ijms25010089.