HAMSAB Diet Ameliorates Dysfunctional Signaling in Pancreatic Islets in Autoimmune Diabetes

Overview

Journal iScience

Publisher Cell Press

Date 2024 Jan 12

PMID 38213620

Authors

Valerie Vandenbempt

Sema Elif Eski

Manoja K Brahma

Ao Li

Javier Negueruela

Ylke Bruggeman

Stephane Demine

Peng Xiao

Alessandra K Cardozo

Nicolas Baeyens

Luciano G Martelotto

Sumeet Pal Singh

Eliana Marino

Conny Gysemans

Esteban N Gurzov

Affiliations

Soon will be listed here.

Abstract

An altered gut microbiota is associated with type 1 diabetes (T1D), affecting the production of short-chain fatty acids (SCFA) and glucose homeostasis. We previously demonstrated that enhancing serum acetate and butyrate using a dietary supplement (HAMSAB) improved glycemia in non-obese diabetic (NOD) mice and patients with established T1D. The effects of SCFA on immune-infiltrated islet cells remain to be clarified. Here, we performed single-cell RNA sequencing on islet cells from NOD mice fed an HAMSAB or control diet. HAMSAB induced a regulatory gene expression profile in pancreas-infiltrated immune cells. Moreover, HAMSAB maintained the expression of β-cell functional genes and decreased cellular stress. HAMSAB-fed mice showed preserved pancreatic endocrine cell identity, evaluated by decreased numbers of poly-hormonal cells. Finally, SCFA increased insulin levels in human β-like cells and improved transplantation outcome in NOD/SCID mice. Our findings support the use of metabolite-based diet as attractive approach to improve glucose control in T1D.

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