The Amplified DNA Logic Gates Based on Aptamer-Receptor Recognition for Cell Detection and Bioimaging

Overview

Journal Biosensors (Basel)

Specialty Biotechnology

Date 2023 Nov 24

PMID 37998143

Authors

Yajing Wang

Di Wu

Xiuping Cao

Yingshu Guo

Affiliations

Soon will be listed here.

Abstract

A powerful and accurate method for identifying and isolating cells would be of great importance due to its sensitivity, gentleness and effectiveness. Here, we designed a receptor-based DNA logic device that allows Boolean logic analysis of multiple cells. For ease of expression, the molecules on the cell surface that can bind to the aptamer are referred to as "receptors". This DNA logic device sends signals based on cell surface sgc8c and sgc4f receptor expression by performing NOT, NOR, AND and OR logic operations, and amplifies and evaluates the signals using HCR. Meanwhile, the release of ICG from the endopore of HMSNs is controlled by affecting structural changes in the DNA logic device. This approach can accurately identify and treat multiple cells on demand based on the presence or absence of cell-specific receptors, facilitating the development of personalized medicine.

Citing Articles

Fluorogenic Aptamer-Based Hybridization Chain Reaction for Signal-Amplified Imaging of Apurinic/Apyrimidinic Endonuclease 1 in Living Cells.

Liu M, Tan Y, Zhou C, Fu Z, Huang R, Li J Biosensors (Basel). 2024; 14(6).

PMID: 38920578 PMC: 11202136. DOI: 10.3390/bios14060274.

References

Chu H, Meng X, Liu B, Liu C, Cheng Y, Sun Z . Supramolecular-mediated dual-functional DNA nanocomposites for programmable cancer therapy. Biomater Sci. 2022; 10(13):3569-3574. DOI: 10.1039/d2bm00323f. View

Li B, Setyawati M, Chen L, Xie J, Ariga K, Lim C . Directing Assembly and Disassembly of 2D MoS Nanosheets with DNA for Drug Delivery. ACS Appl Mater Interfaces. 2017; 9(18):15286-15296. DOI: 10.1021/acsami.7b02529. View

Guo Y, Jing D, Liu S, Yuan Q . Construction of intelligent moving micro/nanomotors and their applications in biosensing and disease treatment. Theranostics. 2023; 13(9):2993-3020. PMC: 10240815. DOI: 10.7150/thno.81845. View

Zadegan R, Jepsen M, Hildebrandt L, Birkedal V, Kjems J . Construction of a fuzzy and Boolean logic gates based on DNA. Small. 2015; 11(15):1811-7. DOI: 10.1002/smll.201402755. View

Duan Y, Glazier R, Bazrafshan A, Hu Y, Rashid S, Petrich B . Mechanically Triggered Hybridization Chain Reaction. Angew Chem Int Ed Engl. 2021; 60(36):19974-19981. PMC: 8390435. DOI: 10.1002/anie.202107660. View

Jiang Q, Yue S, Yu K, Tian T, Zhang J, Chu H . Endogenous microRNA triggered enzyme-free DNA logic self-assembly for amplified bioimaging and enhanced gene therapy via in situ generation of siRNAs. J Nanobiotechnology. 2021; 19(1):288. PMC: 8474761. DOI: 10.1186/s12951-021-01040-x. View

Jiang S, Xiao M, Sun W, Crespy D, Mailander V, Peng X . Synergistic Anticancer Therapy by Ovalbumin Encapsulation-Enabled Tandem Reactive Oxygen Species Generation. Angew Chem Int Ed Engl. 2020; 59(45):20008-20016. PMC: 7693068. DOI: 10.1002/anie.202006649. View

Ren K, Liu Y, Wu J, Zhang Y, Zhu J, Yang M . A DNA dual lock-and-key strategy for cell-subtype-specific siRNA delivery. Nat Commun. 2016; 7:13580. PMC: 5476801. DOI: 10.1038/ncomms13580. View

Guo Y, Cao X, Zheng X, Abbas S, Li J, Tan W . Construction of nanocarriers based on nucleic acids and their applications in nanobiology delivery systems. Natl Sci Rev. 2022; 9(5):nwac006. PMC: 9162387. DOI: 10.1093/nsr/nwac006. View

10.

Douglas S, Bachelet I, Church G . A logic-gated nanorobot for targeted transport of molecular payloads. Science. 2012; 335(6070):831-4. DOI: 10.1126/science.1214081. View

11.

Wang L, Huo M, Chen Y, Shi J . Coordination-Accelerated "Iron Extraction" Enables Fast Biodegradation of Mesoporous Silica-Based Hollow Nanoparticles. Adv Healthc Mater. 2017; 6(22). DOI: 10.1002/adhm.201700720. View

12.

Meng H, Liu H, Kuai H, Peng R, Mo L, Zhang X . Aptamer-integrated DNA nanostructures for biosensing, bioimaging and cancer therapy. Chem Soc Rev. 2016; 45(9):2583-602. DOI: 10.1039/c5cs00645g. View

13.

You M, Zhu G, Chen T, Donovan M, Tan W . Programmable and multiparameter DNA-based logic platform for cancer recognition and targeted therapy. J Am Chem Soc. 2014; 137(2):667-74. PMC: 4308741. DOI: 10.1021/ja509263k. View

14.

Zhang C, Shen L, Liang C, Dong Y, Yang J, Xu J . DNA Sequential Logic Gate Using Two-Ring DNA. ACS Appl Mater Interfaces. 2016; 8(14):9370-6. DOI: 10.1021/acsami.6b00847. View

15.

Ma X, Chen X, Tang Y, Yan R, Miao P . Triple-Input Molecular AND Logic Gates for Sensitive Detection of Multiple miRNAs. ACS Appl Mater Interfaces. 2019; 11(44):41157-41164. DOI: 10.1021/acsami.9b16812. View

16.

Zheng C, Zhang J, Chan H, Hu H, Lv S, Na N . Engineering Nano-Therapeutics to Boost Adoptive Cell Therapy for Cancer Treatment. Small Methods. 2021; 5(5):e2001191. DOI: 10.1002/smtd.202001191. View

17.

Stojanovic M, Mitchell T, Stefanovic D . Deoxyribozyme-based logic gates. J Am Chem Soc. 2002; 124(14):3555-61. DOI: 10.1021/ja016756v. View

18.

Mi Y, Hagan 4th C, Vincent B, Wang A . Emerging Nano-/Microapproaches for Cancer Immunotherapy. Adv Sci (Weinh). 2019; 6(6):1801847. PMC: 6425500. DOI: 10.1002/advs.201801847. View

19.

Chen J, Tang J, Meng H, Liu Z, Wang L, Geng X . Recognition triggered assembly of split aptamers to initiate a hybridization chain reaction for wash-free and amplified detection of exosomes. Chem Commun (Camb). 2020; 56(63):9024-9027. DOI: 10.1039/d0cc02337j. View

20.

Okamoto A, Tanaka K, Saito I . DNA logic gates. J Am Chem Soc. 2004; 126(30):9458-63. DOI: 10.1021/ja047628k. View