Aromatic DNA Adducts in Human Bone Marrow and Peripheral Blood Leukocytes

Overview

Journal Carcinogenesis

Specialty Oncology

Date 1986 Dec 1

PMID 3779901

Citations 20

Authors

D H Phillips

A Hewer

P L Grover

Affiliations

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Abstract

DNA from normal human bone marrow mononuclear and non-mononuclear cells was analysed by 32P-post-labelling for the presence of aromatic adducts. Ten out of 10 individuals showed the presence of adducts at levels of 1-9 adducts per 10(8) nucleotides that were not detected in four samples of human foetal bone marrow. Inter-individual variations in the patterns of these presumed aromatic adducts were observed. Similar adducts were also present in the DNA of peripheral white blood cells of both smokers and non-smokers, although at lower levels than in bone marrow. The data suggest that the adducts result from environmental exposure to an as-yet-unidentified genotoxic agent or agents.

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