NLRP14 Safeguards Calcium Homeostasis Via Regulating the K27 Ubiquitination of Nclx in Oocyte-to-Embryo Transition

Overview

Journal Adv Sci (Weinh)

Specialty Biomedical Engineering

Date 2023 Jul 26

PMID 37493331

Authors

Tie-Gang Meng

Jia-Ni Guo

Liu Zhu

Yike Yin

Feng Wang

Zhi-Ming Han

Lei Lei

Xue-Shan Ma

Yue Xue

Wei Yue

Xiao-Qing Nie

Zheng-Hui Zhao

Hong-Yong Zhang

Si-Min Sun

Ying-Chun Ouyang

Yi Hou

Heide Schatten

Zhenyu Ju

Xiang-Hong Ou

Zhen-Bo Wang

Catherine C L Wong

Zhonghan Li

Qing-Yuan Sun

Affiliations

Soon will be listed here.

Abstract

Sperm-induced Ca rise is critical for driving oocyte activation and subsequent embryonic development, but little is known about how lasting Ca oscillations are regulated. Here it is shown that NLRP14, a maternal effect factor, is essential for keeping Ca oscillations and early embryonic development. Few embryos lacking maternal NLRP14 can develop beyond the 2-cell stage. The impaired developmental potential of Nlrp14-deficient oocytes is mainly caused by disrupted cytoplasmic function and calcium homeostasis due to altered mitochondrial distribution, morphology, and activity since the calcium oscillations and development of Nlrp14-deficient oocytes can be rescued by substitution of whole cytoplasm by spindle transfer. Proteomics analysis reveal that cytoplasmic UHRF1 (ubiquitin-like, containing PHD and RING finger domains 1) is significantly decreased in Nlrp14-deficient oocytes, and Uhrf1-deficient oocytes also show disrupted calcium homeostasis and developmental arrest. Strikingly, it is found that the mitochondrial Na /Ca exchanger (NCLX) encoded by Slc8b1 is significantly decreased in the Nlrp14 oocyte. Mechanistically, NLRP14 interacts with the NCLX intrinsically disordered regions (IDRs) domain and maintain its stability by regulating the K27-linked ubiquitination. Thus, the study reveals NLRP14 as a crucial player in calcium homeostasis that is important for early embryonic development.

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