Mitofusin1 in Oocyte is Essential for Female Fertility

Overview

Journal Redox Biol

Specialties Biology
Cell Biology
Endocrinology

Date 2019 Jan 29

PMID 30690319

Citations 30

Authors

Xiaojing Hou

Shuai Zhu

Hao Zhang

Chunling Li

Danhong Qiu

Juan Ge

Xuejiang Guo

Qiang Wang

Affiliations

Soon will be listed here.

Abstract

Mitofusins (Mfn) are the important regulators of mitochondrial organization in mammalian cells; however, their roles during oocyte development remain unknown. In the present study, we generated mice with oocyte-specific knockout of Mfn1 or Mfn2 (Mfn1;Zp3-Cre or Mfn2;Zp3-Cre). We report that deletion of Mfn1, but not Mfn2, in oocytes leads to female mice sterility, associated with the defective folliculogenesis and impaired oocyte quality. In specific, follicles are arrested at secondary stage in Mfn1;Zp3-Cre mice, accompanying with the reduced proliferation of granulosa cells. Moreover, alterations of mitochondrial structure and distribution pattern are readily observed in Mfn1-null oocytes. Consistent with this, mitochondrial activity and function are severely disrupted in oocytes from Mfn1;Zp3-Cre mice. In addition, the differentially expressed genes in Mfn1-deleted oocytes are also identified by whole-transcriptome sequencing. In sum, these results demonstrate that Mfn1-modulated mitochondrial function is essential for oocyte development and folliculogenesis, providing a novel mechanism determining female fertility.

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