Cysteine-rich Intestinal Protein 1 is a Novel Surface Marker for Human Myometrial Stem/progenitor Cells

Overview

Journal Commun Biol

Specialty Biology

Date 2023 Jul 3

PMID 37400623

Authors

Emmanuel N Paul

Tyler J Carpenter

Sarah Fitch

Rachael Sheridan

Kin H Lau

Ripla Arora

Jose M Teixeira

Affiliations

Soon will be listed here.

Abstract

Myometrial stem/progenitor cells (MyoSPCs) have been proposed as the cells of origin for uterine fibroids, but the identity of the MyoSPC has not been well established. We previously identified SUSD2 as a possible MyoSPC marker, but the relatively poor enrichment in stem cell characteristics of SUSD2+ over SUSD2- cells compelled us to find better markers. We combined bulk RNA-seq of SUSD2+/- cells with single cell RNA-seq to identify markers for MyoSPCs. We observed seven distinct cell clusters within the myometrium, with the vascular myocyte cluster most highly enriched for MyoSPC characteristics and markers. CRIP1 expression was found highly upregulated by both techniques and was used as a marker to sort CRIP1+/PECAM1- cells that were both enriched for colony forming potential and able to differentiate into mesenchymal lineages, suggesting that CRIP1+/PECAM1- cells could be used to better study the etiology of uterine fibroids.

Citing Articles

HMGA2 overexpression induces plasticity in myometrial cells and a transcriptomic profile more similar to that of uterine fibroids.

Paul E, Carpenter T, Pavliscak L, Bennett A, Ochoa-Bernal M, Fazleabas A F S Sci. 2024; 5(4):369-378.

PMID: 39025326 PMC: 11588543. DOI: 10.1016/j.xfss.2024.07.006.

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