The Environmental Stress Response Regulates Ribosome Content in Cell Cycle-arrested

Overview

Journal Front Cell Dev Biol

Specialty Cell Biology

Date 2023 May 1

PMID 37123399

Authors

Allegra Terhorst

Arzu Sandikci

Charles A Whittaker

Tamas Szoradi

Liam J Holt

Gabriel E Neurohr

Angelika Amon

Affiliations

Soon will be listed here.

Abstract

Prolonged cell cycle arrests occur naturally in differentiated cells and in response to various stresses such as nutrient deprivation or treatment with chemotherapeutic agents. Whether and how cells survive prolonged cell cycle arrests is not clear. Here, we used to compare physiological cell cycle arrests and genetically induced arrests in G1-, meta- and anaphase. Prolonged cell cycle arrest led to growth attenuation in all studied conditions, coincided with activation of the Environmental Stress Response (ESR) and with a reduced ribosome content as determined by whole ribosome purification and TMT mass spectrometry. Suppression of the ESR through hyperactivation of the Ras/PKA pathway reduced cell viability during prolonged arrests, demonstrating a cytoprotective role of the ESR. Attenuation of cell growth and activation of stress induced signaling pathways also occur in arrested human cell lines, raising the possibility that the response to prolonged cell cycle arrest is conserved.

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