Increased Mesoscale Diffusivity in Response to Acute Glucose Starvation

Overview

Journal MicroPubl Biol

Specialty Biology

Date 2023 Mar 13

PMID 36908311

Authors

Ying Xie

David Gresham

Liam J Holt

Affiliations

Soon will be listed here.

Abstract

Macromolecular crowding is an important property of cells that impacts multiple biological processes. Passive microrheology using single particle tracking is a powerful means of studying macromolecular crowding. Here we monitored the diffusivity of self-assembling fluorescent nanoparticles (μNS) and mRNPs ( -PP7) in response to acute glucose starvation. mRNP diffusivity was reduced upon glucose starvation as previously reported. By contrast, we observed increased diffusivity of μNS particles. Our results suggest that, upon glucose starvation, mRNP granule diffusivity may be reduced due to increased physical interactions, whereas macromolecular crowding in the cytoplasm may be globally reduced.

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