Assay of Tryptophan Decarboxylase from Catharanthus Roseus Plant Cell Cultures by High-performance Liquid Chromatography

Overview

Journal Anal Biochem

Publisher Elsevier

Specialty Biochemistry

Date 1987 Aug 15

PMID 3688427

Citations 10

Authors

E J Pennings

I Hegger

R van der Heijden

J A Duine

R Verpoorte

Affiliations

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Abstract

An assay is described for the enzyme tryptophan decarboxylase from plant cell suspension cultures. It is based on the fluorometric detection of tryptamine by HPLC on a LiChrosorb RP-8 Select B column. Tryptophan decarboxylase from Catharanthus roseus was induced by transferring 14-day-old cells into an induction medium. Optimum activity was found 2 days after transfer, the increase being 5- to 10-fold. When kept at -15 degrees C the crude enzyme lost half its activity in about 7 days. The rate of the decarboxylation reaction was linear for at least 3 h at 35 degrees C.

Citing Articles

Subcellular localization of tryptophan decarboxylase, strictosidine synthase and strictosidine glucosidase in suspension cultured cells of Catharanthus roseus and Tabernaemontana divaricata.

Stevens L, Blom T, Verpoorte R Plant Cell Rep. 2013; 12(10):573-6.

PMID: 24201788 DOI: 10.1007/BF00233063.

Elicitor-mediated induction of isochorismate synthase and accumulation of 2,3-dihydroxy benzoic acid in Catharanthus roseus cell suspension and shoot cultures.

Moreno P, van der Heijden R, Verpoorte R Plant Cell Rep. 2013; 14(2-3):188-91.

PMID: 24192892 DOI: 10.1007/BF00233788.

The role of aromatic L-amino acid decarboxylase in bacillamide C biosynthesis by Bacillus atrophaeus C89.

Yuwen L, Zhang F, Chen Q, Lin S, Zhao Y, Li Z Sci Rep. 2013; 3:1753.

PMID: 23628927 PMC: 3639450. DOI: 10.1038/srep01753.

Tryptophan decarboxylase plays an important role in ajmalicine biosynthesis in Rauvolfia verticillata.

Liu W, Chen R, Chen M, Zhang H, Peng M, Yang C Planta. 2012; 236(1):239-50.

PMID: 22331368 DOI: 10.1007/s00425-012-1608-z.

Targeting tryptophan decarboxylase to selected subcellular compartments of tobacco plants affects enzyme stability and in vivo function and leads to a lesion-mimic phenotype.

Di Fiore S, Li Q, Leech M, Schuster F, Emans N, Fischer R Plant Physiol. 2002; 129(3):1160-9.

PMID: 12114570 PMC: 166510. DOI: 10.1104/pp.010889.