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A Chimaeric Tryptophan Decarboxylase Gene As a Novel Selectable Marker in Plant Cells

Overview
Journal Plant Mol Biol
Date 1993 Aug 1
PMID 8358036
Citations 8
Authors
O J Goddijn
P M van der Duyn Schouten
R A Schilperoort
J H Hoge
Affiliations
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Abstract

A novel selection system for plant genetic transformation was developed based on the enzyme tryptophan decarboxylase (TDC; EC 4.1.1.28) from Catharanthus roseus. This enzyme converts the toxic tryptophan analogue 4-methyl tryptophan (4-mT) into the non-toxic compound 4-methyl tryptamine. Expression of tdc in transgenic plants that have no endogenous TDC-activity allows selection on 4-mT. A vector was constructed containing a tdc cDNA clone under control of the constitutively expressed cauliflower mosaic virus 35S promoter. This vector was used in Agrobacterium-mediated tobacco leaf disc transformation experiments. The optimal concentration for selection with 4-mT was found to be 0.1 mM. The transformed nature of shoots obtained after tdc gene transfer and subsequent selection on 0.1 mM 4-mT was confirmed by northern blot analysis.

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References
1.
Marsh J, Erfle M, Wykes E . The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene. 1984; 32(3):481-5. DOI: 10.1016/0378-1119(84)90022-2. View
2.
Pennings E, Hegger I, van der Heijden R, Duine J, Verpoorte R . Assay of tryptophan decarboxylase from Catharanthus roseus plant cell cultures by high-performance liquid chromatography. Anal Biochem. 1987; 165(1):133-6. DOI: 10.1016/0003-2697(87)90210-7. View
3.
Noe W, Mollenschott C, Berlin J . Tryptophan decarboxylase from Catharanthus roseus cell suspension cultures: purification, molecular and kinetic data of the homogenous protein. Plant Mol Biol. 2013; 3(5):281-8. DOI: 10.1007/BF00017782. View
4.
Bevan M . Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res. 1984; 12(22):8711-21. PMC: 320409. DOI: 10.1093/nar/12.22.8711. View
5.
Mattanovich D, Ruker F, Machado A, Laimer M, Regner F, Steinkellner H . Efficient transformation of Agrobacterium spp. by electroporation. Nucleic Acids Res. 1989; 17(16):6747. PMC: 318385. DOI: 10.1093/nar/17.16.6747. View