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Elicitor-mediated Induction of Isochorismate Synthase and Accumulation of 2,3-dihydroxy Benzoic Acid in Catharanthus Roseus Cell Suspension and Shoot Cultures

Overview
Journal Plant Cell Rep
Publisher Springer
Date 2013 Nov 7
PMID 24192892
Citations 10
Authors
P R Moreno
R van der Heijden
R Verpoorte
Affiliations
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Abstract

After elicitation, cell suspension cultures of Catharanthus roseus accumulate phenolic compounds. The major phenolic compound produced was isolated and identified as 2,3-dihydroxybenzoic acid (DHBA). The accumulation of this compound is a rapid response to the addition of elicitor; within 6 h after the addition of elicitor, DHBA concentration reached 6.3 mg/l cell suspension. DHBA was not detected in non-elicited cells. The formation of DHBA in elicited cells was correlated with the induction of the enzyme isochorismate synthase (ICS). Shoot cultures of C. roseus also presented a strong induction of ICS after elicitation. Due to its biological activity, DHBA could play a role in the defence mechanism of C. roseus.

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