Subcellular Localization of Tryptophan Decarboxylase, Strictosidine Synthase and Strictosidine Glucosidase in Suspension Cultured Cells of Catharanthus Roseus and Tabernaemontana Divaricata

Overview

Journal Plant Cell Rep

Publisher Springer

Date 2013 Nov 9

PMID 24201788

Citations 22

Authors

L H Stevens

T J Blom

R Verpoorte

Affiliations

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Abstract

The subcellular localization of tryptophan decarboxylase, strictosidine synthase and strictosidine glucosidase in suspension cultured cells of Catharanthus roseus (L.) G. Don and Tabernaemontana divaricata (L.) R. Br. ex Roem. et Schult, was investigated. It was found that tryptophan decarboxylase is an extra-vacuolar enzyme, whereas strictosidine synthase is active inside the vacuole. Strong indications were obtained for the localization of strictosidine glucosidase on the outside of the tonoplast. The results suggest that tryptamine is transported into the vacuole where it is condensed with secologanin to form strictosidine, and that strictosidine passes the tonoplast and is subsequently hydrolysed outside the vacuole.

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