Integration of Whole Transcriptome Spatial Profiling with Protein Markers

Overview

Journal Nat Biotechnol

Specialty Biotechnology

Date 2023 Jan 2

PMID 36593397

Authors

Nir Ben-Chetrit

Xiang Niu

Ariel D Swett

Jesus Sotelo

Maria S Jiao

Caitlin M Stewart

Catherine Potenski

Paulius Mielinis

Patrick Roelli

Marlon Stoeckius

Dan A Landau

Affiliations

Soon will be listed here.

Abstract

Spatial transcriptomics and proteomics provide complementary information that independently transformed our understanding of complex biological processes. However, experimental integration of these modalities is limited. To overcome this, we developed Spatial PrOtein and Transcriptome Sequencing (SPOTS) for high-throughput simultaneous spatial transcriptomics and protein profiling. Compared with unimodal measurements, SPOTS substantially improves signal resolution and cell clustering and enhances the discovery power in differential gene expression analysis across tissue regions.

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