Multiplexed Ion Beam Imaging of Human Breast Tumors

Overview

Journal Nat Med

Specialties General Medicine
Molecular Biology

Date 2014 Mar 4

PMID 24584119

Citations 553

Authors

Michael Angelo

Sean C Bendall

Rachel Finck

Matthew B Hale

Chuck Hitzman

Alexander D Borowsky

Richard M Levenson

John B Lowe

Scot D Liu

Shuchun Zhao

Yasodha Natkunam

Garry P Nolan

Affiliations

Soon will be listed here.

Abstract

Immunohistochemistry (IHC) is a tool for visualizing protein expression that is employed as part of the diagnostic workup for the majority of solid tissue malignancies. Existing IHC methods use antibodies tagged with fluorophores or enzyme reporters that generate colored pigments. Because these reporters exhibit spectral and spatial overlap when used simultaneously, multiplexed IHC is not routinely used in clinical settings. We have developed a method that uses secondary ion mass spectrometry to image antibodies tagged with isotopically pure elemental metal reporters. Multiplexed ion beam imaging (MIBI) is capable of analyzing up to 100 targets simultaneously over a five-log dynamic range. Here, we used MIBI to analyze formalin-fixed, paraffin-embedded human breast tumor tissue sections stained with ten labels simultaneously. The resulting data suggest that MIBI can provide new insights into disease pathogenesis that will be valuable for basic research, drug discovery and clinical diagnostics.

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