Oligopeptide Sortase Inhibitor Modulates Cell Adhesion and Biofilm Formation

Overview

Journal Antibiotics (Basel)

Specialty Pharmacology

Date 2022 Dec 23

PMID 36551492

Authors

Svetlana A Bozhkova

Ekaterina M Gordina

Dmitry V Labutin

Konstantin V Kudryavtsev

Affiliations

Soon will be listed here.

Abstract

Prevention of bacterial adhesion is one of the most important antivirulence strategies for meeting the global challenge posed by antimicrobial resistance. We aimed to investigate the influence of a peptidic sortase A inhibitor on bacterial adhesion to eukaryotic cells and biofilm formation as a potential method for reducing virulence. The pentapeptide LPRDA was synthesized and characterized as a pure individual organic compound. Incubation of MSSA and MRSA strains with LPRDA induced a subsequent reduction in staphylococcal adhesion to Vero cells and biofilm formation, as visualized by microscopic and spectrophotometric methods, respectively. LPRDA did not have a cytotoxic effect on eukaryotic or bacterial cells. The pentapeptide LPRDA deserves further investigation using in vitro and in vivo models of Gram-positive bacteriemia as a potential antibacterial agent with an antiadhesive mechanism of action.

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