Oligopeptide Targeting Sortase A As Potential Anti-infective Therapy for

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2018 Mar 2

PMID 29491861

Citations 27

Authors

Jianfeng Wang

Hongen Li

Juan Pan

Jing Dong

Xuan Zhou

Xiaodi Niu

Xuming Deng

Affiliations

Soon will be listed here.

Abstract

Sortase A (SrtA)-catalyzed anchorage of surface proteins in most Gram-positive bacteria is indispensable for their virulence, suggesting that this transpeptidase is a promising target for antivirulence therapy. Here, an oligopeptide, LPRDA, was identified as an effective inhibitor of SrtA via virtual screening based on the LPXTG substrate sequence, and it was found to inhibit SrtA activity and (IC = 10.61 μM) by competitively occupying the active site of SrtA. Further, the oligopeptide treatment had no anti- activity, but it provided protection against -induced mastitis in a mouse model. These findings indicate that the oligopeptide could be used as an effective anti-infective agent for the treatment of infection caused by or other Gram-positive bacteria via the targeting of SrtA.

Citing Articles

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Ensemble Docking as a Tool for the Rational Design of Peptidomimetic Sortase A Inhibitors.

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