Graphene-Based Sensor for the Detection of Cortisol for Stress Level Monitoring and Diagnostics

Overview

Journal Diagnostics (Basel)

Specialty Radiology

Date 2022 Nov 11

PMID 36359436

Authors

Alexei Zubarev

Marina Cuzminschi

Ana-Maria Iordache

Stefan-Marian Iordache

Constantin Rizea

Cristiana E A Grigorescu

Carmen Giuglea

Affiliations

Soon will be listed here.

Abstract

In this work, we study the sensing properties of multi-layer graphene combined with pyrrole in order to elaborate low-cost, high-sensitive material for cortisol detection. Graphene nanoplatelets and pyrrole were dispersed in a solution containing 1M HNO by using a powerful ultrasound probe for 10 min, then centrifuged for 30 min at 4000 rpm; polymerization was performed by cyclic voltammetry. The graphene-pyrrole composite was tested to ultra-low levels of cortisol in artificial saliva, consistent to the levels excreted in human salivary samples. The composite was further investigated by Raman spectroscopy and we modeled the interaction between the sensitive layer and cortisol using MarvinBeans software. It shows a good sensitivity for salivary values of cortisol cyclic voltammetry being able to detect a level down to 0.5 ng/mL cortisol.

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References

Wu K, Fei J, Hu S . Simultaneous determination of dopamine and serotonin on a glassy carbon electrode coated with a film of carbon nanotubes. Anal Biochem. 2003; 318(1):100-6. DOI: 10.1016/s0003-2697(03)00174-x. View

Lange T, Dimitrov S, Born J . Effects of sleep and circadian rhythm on the human immune system. Ann N Y Acad Sci. 2010; 1193:48-59. DOI: 10.1111/j.1749-6632.2009.05300.x. View

Kannan S, Ambrose B, Sudalaimani S, Pandiaraj M, Giribabu K, Kathiresan M . A review on chemical and electrochemical methodologies for the sensing of biogenic amines. Anal Methods. 2020; 12(27):3438-3453. DOI: 10.1039/d0ay00358a. View

Richardson S, Shaffer J, Falzon L, Krupka D, Davidson K, Edmondson D . Meta-analysis of perceived stress and its association with incident coronary heart disease. Am J Cardiol. 2012; 110(12):1711-6. PMC: 3511594. DOI: 10.1016/j.amjcard.2012.08.004. View

Alvarez-Martos I, Ferapontova E . Electrochemical Label-Free Aptasensor for Specific Analysis of Dopamine in Serum in the Presence of Structurally Related Neurotransmitters. Anal Chem. 2016; 88(7):3608-16. DOI: 10.1021/acs.analchem.5b04207. View

Yu M, Wu L, Miao J, Wei W, Liu A, Liu S . Titanium dioxide and polypyrrole molecularly imprinted polymer nanocomposites based electrochemical sensor for highly selective detection of p-nonylphenol. Anal Chim Acta. 2019; 1080:84-94. DOI: 10.1016/j.aca.2019.06.053. View

Ohno I . Neuropsychiatry phenotype in asthma: Psychological stress-induced alterations of the neuroendocrine-immune system in allergic airway inflammation. Allergol Int. 2017; 66S:S2-S8. DOI: 10.1016/j.alit.2017.06.005. View

Sekar M, Pandiaraj M, Bhansali S, Ponpandian N, Viswanathan C . Carbon fiber based electrochemical sensor for sweat cortisol measurement. Sci Rep. 2019; 9(1):403. PMC: 6344552. DOI: 10.1038/s41598-018-37243-w. View

Xu G, Jarjes Z, Wang H, Phillips A, Kilmartin P, Travas-Sejdic J . Detection of Neurotransmitters by Three-Dimensional Laser-Scribed Graphene Grass Electrodes. ACS Appl Mater Interfaces. 2018; 10(49):42136-42145. DOI: 10.1021/acsami.8b16692. View

10.

Hewagalamulage S, Lee T, Clarke I, Henry B . Stress, cortisol, and obesity: a role for cortisol responsiveness in identifying individuals prone to obesity. Domest Anim Endocrinol. 2016; 56 Suppl:S112-20. DOI: 10.1016/j.domaniend.2016.03.004. View

11.

Martin P . Stress and Primary Headache: Review of the Research and Clinical Management. Curr Pain Headache Rep. 2016; 20(7):45. DOI: 10.1007/s11916-016-0576-6. View

12.

Lee S, Oh J, Kim D, Piao Y . A sensitive electrochemical sensor using an iron oxide/graphene composite for the simultaneous detection of heavy metal ions. Talanta. 2016; 160:528-536. DOI: 10.1016/j.talanta.2016.07.034. View

13.

Kundu T, Pramanik A . Expeditious and eco-friendly synthesis of new multifunctionalized pyrrole derivatives and evaluation of their antioxidant property. Bioorg Chem. 2020; 98:103734. DOI: 10.1016/j.bioorg.2020.103734. View

14.

Kim K, Park C, Park S, Kim J, Seo S, An J . In-situ food spoilage monitoring using a wireless chemical receptor-conjugated graphene electronic nose. Biosens Bioelectron. 2021; 200:113908. DOI: 10.1016/j.bios.2021.113908. View

15.

Bemis H, Yarboi J, Gerhardt C, Vannatta K, Desjardins L, Murphy L . Childhood Cancer in Context: Sociodemographic Factors, Stress, and Psychological Distress Among Mothers and Children. J Pediatr Psychol. 2015; 40(8):733-43. PMC: 4536846. DOI: 10.1093/jpepsy/jsv024. View

16.

Song H, Fang F, Tomasson G, Arnberg F, Mataix-Cols D, Fernandez de la Cruz L . Association of Stress-Related Disorders With Subsequent Autoimmune Disease. JAMA. 2018; 319(23):2388-2400. PMC: 6583688. DOI: 10.1001/jama.2018.7028. View

17.

Syama S, Mohanan P . Safety and biocompatibility of graphene: A new generation nanomaterial for biomedical application. Int J Biol Macromol. 2016; 86:546-55. DOI: 10.1016/j.ijbiomac.2016.01.116. View

18.

Xu Y, Cheng Y, Jia Y, Ye B . Synthesis of MOF-derived Ni@C materials for the electrochemical detection of histamine. Talanta. 2020; 219:121360. DOI: 10.1016/j.talanta.2020.121360. View

19.

Marie M, Mandal S, Manasreh O . An Electrochemical Glucose Sensor Based on Zinc Oxide Nanorods. Sensors (Basel). 2015; 15(8):18714-23. PMC: 4570342. DOI: 10.3390/s150818714. View

20.

Trchova M, Stejskal J . Resonance Raman Spectroscopy of Conducting Polypyrrole Nanotubes: Disordered Surface versus Ordered Body. J Phys Chem A. 2018; 122(48):9298-9306. DOI: 10.1021/acs.jpca.8b09794. View