Early Developmental Plasticity Enables the Induction of an Intermediate Extraembryonic Cell State

Overview

Journal Sci Adv

Specialties Biology
Science

Date 2022 Nov 4

PMID 36332016

Authors

Anusha Sathyanarayanan

Elizabeth Ing-Simmons

Rui Chen

Hyun-Woo Jeong

Hatice O Ozguldez

Rui Fan

Binyamin Duethorn

Kee-Pyo Kim

Yung Su Kim

Martin Stehling

Heike Brinkmann

Hans R Scholer

Ralf H Adams

Juan M Vaquerizas

Ivan Bedzhov

Affiliations

Soon will be listed here.

Abstract

Two fundamental elements of pre-implantation embryogenesis are cells' intrinsic self-organization program and their developmental plasticity, which allows embryos to compensate for alterations in cell position and number; yet, these elements are still poorly understood. To be able to decipher these features, we established culture conditions that enable the two fates of blastocysts' extraembryonic lineages-the primitive endoderm and the trophectoderm-to coexist. This plasticity emerges following the mechanisms of the first lineage segregation in the mouse embryo, and it manifests as an extended potential for extraembryonic chimerism during the pre-implantation embryogenesis. Moreover, this shared state enables robust assembly into higher-order blastocyst-like structures, thus combining both the cell fate plasticity and self-organization features of the early extraembryonic lineages.

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