Analyzing Embryo Dormancy at Single-cell Resolution Reveals Dynamic Transcriptional Responses and Activation of Integrin-Yap/Taz Prosurvival Signaling

Overview

Journal Cell Stem Cell

Publisher Cell Press

Specialty Cell Biology

Date 2024 Jul 24

PMID 39047740

Authors

Rui Chen

Rui Fan

Fei Chen

Niraimathi Govindasamy

Heike Brinkmann

Martin Stehling

Ralf H Adams

Hyun-Woo Jeong

Ivan Bedzhov

Affiliations

Soon will be listed here.

Abstract

Embryonic diapause is a reproductive adaptation that enables some mammalian species to halt the otherwise continuous pace of embryonic development. In this dormant state, the embryo exploits poorly understood regulatory mechanisms to preserve its developmental potential for prolonged periods of time. Here, using mouse embryos and single-cell RNA sequencing, we molecularly defined embryonic diapause at single-cell resolution, revealing transcriptional dynamics while the embryo seemingly resides in a state of suspended animation. Additionally, we found that the dormant pluripotent cells rely on integrin receptors to sense their microenvironment and preserve their viability via Yap/Taz-mediated prosurvival signaling.

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