Live Slow-frozen Human Tumor Tissues Viable for 2D, 3D, Ex Vivo Cultures and Single-cell RNAseq

Overview

Journal Commun Biol

Specialty Biology

Date 2022 Oct 28

PMID 36307545

Authors

Gaetana Restivo

Aizhan Tastanova

Zsolt Balazs

Federica Panebianco

Maren Diepenbruck

Caner Ercan

Bodgan-T Preca

Jurg Hafner

Walter P Weber

Christian Kurzeder

Marcus Vetter

Simone Munst Soysal

Christian Beisel

Mohamed Bentires-Alj

Salvatore Piscuoglio

Michael Krauthammer

Mitchell P Levesque

Affiliations

Soon will be listed here.

Abstract

Biobanking of surplus human healthy and disease-derived tissues is essential for diagnostics and translational research. An enormous amount of formalin-fixed and paraffin-embedded (FFPE), Tissue-Tek OCT embedded or snap-frozen tissues are preserved in many biobanks worldwide and have been the basis of translational studies. However, their usage is limited to assays that do not require viable cells. The access to intact and viable human material is a prerequisite for translational validation of basic research, for novel therapeutic target discovery, and functional testing. Here we show that surplus tissues from multiple solid human cancers directly slow-frozen after resection can subsequently be used for different types of methods including the establishment of 2D, 3D, and ex vivo cultures as well as single-cell RNA sequencing with similar results when compared to freshly analyzed material.

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