RNase H1, the Gold Standard for R-Loop Detection

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2022 Jun 15

PMID 35704187

Authors

Susana M Cerritelli

Kiran Sakhuja

Robert J Crouch

Affiliations

Soon will be listed here.

Abstract

RNase H1 has become an essential tool to uncover the physiological and pathological roles of R-loops, three-stranded structures consisting of and RNA-DNA hybrid opposite to a single DNA strand (ssDNA). RNase H1 degrades the RNA portion of the R-loops returning the two DNA strands to double-stranded form (dsDNA). Overexpression of RNase H1 in different systems has helped to address the questions of where R-loops are located, their abundance, and mechanisms of formation, stability, and degradation. In this chapter we review multiple studies that used RNase H1 as an instrument to investigate R-loops multiple functions and their relevance in health and diseases.

Citing Articles

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