Optogenetic Actuator - ERK Biosensor Circuits Identify MAPK Network Nodes That Shape ERK dynamics

Overview

Journal Mol Syst Biol

Specialty Molecular Biology

Date 2022 Jun 13

PMID 35694820

Authors

Coralie Dessauges

Jan Mikelson

Maciej Dobrzynski

Marc-Antoine Jacques

Agne Frismantiene

Paolo Armando Gagliardi

Mustafa Khammash

Olivier Pertz

Affiliations

Soon will be listed here.

Abstract

Combining single-cell measurements of ERK activity dynamics with perturbations provides insights into the MAPK network topology. We built circuits consisting of an optogenetic actuator to activate MAPK signaling and an ERK biosensor to measure single-cell ERK dynamics. This allowed us to conduct RNAi screens to investigate the role of 50 MAPK proteins in ERK dynamics. We found that the MAPK network is robust against most node perturbations. We observed that the ERK-RAF and the ERK-RSK2-SOS negative feedback operate simultaneously to regulate ERK dynamics. Bypassing the RSK2-mediated feedback, either by direct optogenetic activation of RAS, or by RSK2 perturbation, sensitized ERK dynamics to further perturbations. Similarly, targeting this feedback in a human ErbB2-dependent oncogenic signaling model increased the efficiency of a MEK inhibitor. The RSK2-mediated feedback is thus important for the ability of the MAPK network to produce consistent ERK outputs, and its perturbation can enhance the efficiency of MAPK inhibitors.

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