Bright Monomeric Near-infrared Fluorescent Proteins As Tags and Biosensors for Multiscale Imaging

Overview

Journal Nat Commun

Specialty Biology

Date 2016 Aug 20

PMID 27539380

Citations 140

Authors

Daria M Shcherbakova

Mikhail Baloban

Alexander V Emelyanov

Michael Brenowitz

Peng Guo

Vladislav V Verkhusha

Affiliations

Soon will be listed here.

Abstract

Monomeric near-infrared (NIR) fluorescent proteins (FPs) are in high demand as protein tags and components of biosensors for deep-tissue imaging and multicolour microscopy. We report three bright and spectrally distinct monomeric NIR FPs, termed miRFPs, engineered from bacterial phytochrome, which can be used as easily as GFP-like FPs. miRFPs are 2-5-fold brighter in mammalian cells than other monomeric NIR FPs and perform well in protein fusions, allowing multicolour structured illumination microscopy. miRFPs enable development of several types of NIR biosensors, such as for protein-protein interactions, RNA detection, signalling cascades and cell fate. We demonstrate this by engineering the monomeric fluorescence complementation reporters, the IκBα reporter for NF-κB pathway and the cell cycle biosensor for detection of proliferation status of cells in culture and in animals. miRFPs allow non-invasive visualization and detection of biological processes at different scales, from super-resolution microscopy to in vivo imaging, using the same probes.

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