Increased Expression Enhances SARS-CoV-1 and SARS-CoV-2 Production in Vero-Derived Transgenic Cell Lines

Overview

Journal Mol Biol

Specialties Biochemistry
Biology

Date 2022 Jun 13

PMID 35693978

Authors

A A Dolskiy

I V Grishchenko

S A Bodnev

A A Nazarenko

A M Smirnova

A K Matveeva

L E Bulychev

A S Ovchinnikova

T V Tregubchak

A V Zaykovskaya

I R Imatdinov

O V Pyankov

E V Gavrilova

R A Maksyutov

D V Yudkin

Affiliations

Soon will be listed here.

Abstract

Coronaviridae is a family of single-stranded RNA (ssRNA) viruses that can cause diseases with high mortality rates. SARS-CoV-1 and MERS-CoV appeared in 2002‒2003 and 2012, respectively. A novel coronavirus, SARS-CoV-2, emerged in 2019 in Wuhan (China) and has caused more than 5 million deaths in worldwide. The entry of SARS-CoV-1 into the cell is due to the interaction of the viral spike (S) protein and the cell protein, angiotensin-converting enzyme 2 (ACE2). After infection, virus assembly occurs in Golgi apparatus-derived vesicles during exocytosis. One of the possible participants in this process is LAMP1 protein. We established transgenic Vero cell lines with increased expression of human gene and evaluated SARS-CoV-1 and SARS-CoV-2 production. An increase in the production of both viruses in -expressing cells when compared with Vero cells was observed, especially in the presence of trypsin during infection. From these results it can be assumed that LAMP1 promotes SARS-CoV-1 and SARS-CoV-2 production due to enhanced exocytosis.

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