The Life Cycle of SARS Coronavirus in Vero E6 Cells

Overview

Journal J Med Virol

Specialty Microbiology

Date 2004 Jun 2

PMID 15170625

Citations 71

Authors

Zhang Qinfen

Cui Jinming

Huang Xiaojun

Zheng Huanying

Huang Jicheng

Fang Ling

Li Kunpeng

Zhang Jingqiang

Affiliations

Soon will be listed here.

Abstract

The aim of the study was to establish the life cycle of severe acute respiratory syndrome-associated coronavirus (SARS CoV) in host cells and determine the pathogenesis of SARS. Vero E6 cells (African green monkey kidney cells) were inoculated with SARS coronavirus for 3, 7, 24, 48, and 72 hr, respectively, and were observed under electron microscope. It was found that the SARS coronavirus entered the cells through membrane fusion instead of endocytosis, and then the nucleocapsids assembled in the RER and matured by budding into the smooth vesicles, which were derived from the Golgi apparatus. The smooth vesicles fused with the cell membrane, and the mature particles were released. A special phenomenon was that some virus-like particles appeared in the nucleus. We propose a scheme of the life cycle of SARS coronavirus and discuss the mechanism of its replication in Vero E6 cells.

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