Ultrasensitive Detection of SARS-CoV-2 RNA and Antigen Using Single-molecule Optofluidic Chip
Overview
Affiliations
Nucleic acids and proteins are the two most important target types used in molecular diagnostics. In many instances, simultaneous sensitive and accurate detection of both biomarkers from the same sample would be desirable, but standard detection methods are highly optimized for one type and not cross-compatible. Here, we report the simultaneous multiplexed detection of SARS-CoV-2 RNAs and antigens with single molecule sensitivity. Both analytes are isolated and labeled using a single bead-based solid-phase extraction protocol, followed by fluorescence detection on a multi-channel optofluidic waveguide chip. Direct amplification-free detection of both biomarkers from nasopharyngeal swab samples is demonstrated with single molecule detection sensitivity, opening the door for ultrasensitive dual-target analysis in infectious disease diagnosis, oncology, and other applications.
Emerging nanophotonic biosensor technologies for virus detection.
Bannur Nanjunda S, Seshadri V, Krishnan C, Rath S, Arunagiri S, Bao Q Nanophotonics. 2024; 11(22):5041-5059.
PMID: 39634299 PMC: 11501160. DOI: 10.1515/nanoph-2022-0571.
Escobar A, Diab-Liu A, Bosland K, Xu C Biosensors (Basel). 2023; 13(10).
PMID: 37887128 PMC: 10605122. DOI: 10.3390/bios13100935.
Machine learning at the edge for AI-enabled multiplexed pathogen detection.
Ganjalizadeh V, Meena G, Stott M, Hawkins A, Schmidt H Sci Rep. 2023; 13(1):4744.
PMID: 36959357 PMC: 10034896. DOI: 10.1038/s41598-023-31694-6.
Konoplev G, Agafonova D, Bakhchova L, Mukhin N, Kurachkina M, Schmidt M Biomedicines. 2022; 10(2).
PMID: 35203416 PMC: 8868674. DOI: 10.3390/biomedicines10020207.
FPGA Integrated Optofluidic Biosensor for Real-Time Single Biomarker Analysis.
Sampad M, Amin M, Hawkins A, Schmidt H IEEE Photonics J. 2021; 14(1).
PMID: 34900090 PMC: 8658630. DOI: 10.1109/jphot.2021.3127484.