Combination of Serological Total Antibody and RT-PCR Test for Detection of SARS-COV-2 Infections

Overview

Journal J Virol Methods

Specialty Microbiology

Date 2020 Jun 20

PMID 32554043

Citations 36

Authors

Pei Wang

Affiliations

Soon will be listed here.

Abstract

The purpose of this study was to investigate the feasibility of serological total antibody tests combined with RT-PCR for detection of SARS-COV-2. We conducted a retrospective study in which 375 patients were enrolled during the outbreak of SARS-COV-2 from 25th January to 16th March 2020. Patients were divided into a COVID-19 group (n = 141) and a control group (n = 234). Serum samples and throat swabs were collected from 375 patients for total antibody testing against SARS-COV-2 and RT-PCR analysis, respectively. The results indicated that diagnostic sensitivity and specificity were 95.7 % and 98.7 %, 92.2 % and 100 % by total antibody tests and RT-PCR, respectively. The sensitivity and specificity of total antibody tests combined with RT-PCR were 98.6 % and 98.7 %. The sensitivity of the combined method was significantly higher than RT-PCR (X = 5.16, P < 0.05), and similar to that of total antibody tests (X = 1.15, P> 0.05). This study supported the advantage of the combined method for detection of SARS-COV-2 with a high degree of sensitivity and specificity, as a useful tool for accurate diagnosis and timely treatment of suspected patients, epidemiological investigation, as well as monitoring ongoing outbreaks of infections with SARS-COV-2.

Citing Articles

Anti-SARS-CoV-2 IgM Antibody Levels Measured by an In-House ELISA in a Convalescent Latin Population Persist over Time and Exhibit Neutralizing Capacity to Several Variants of Concern.

Espino A, Armina-Rodriguez A, Cardona P, Ocasio-Malave C, Alvarez L, Sariol C Diagnostics (Basel). 2024; 14(19).

PMID: 39410613 PMC: 11475847. DOI: 10.3390/diagnostics14192209.

Respiratory virus circulation during the first year of the COVID-19 pandemic in the Household Influenza Vaccine Evaluation (HIVE) cohort.

Fine S, Bazzi L, Callear A, Petrie J, Malosh R, Foster-Tucker J Influenza Other Respir Viruses. 2023; 17(3):e13106.

PMID: 36875204 PMC: 9975790. DOI: 10.1111/irv.13106.

The correlation between IgM and IgG antibodies with blood profile in patients infected with severe acute respiratory syndrome coronavirus.

Alibolandi Z, Ostadian A, Sayyah S, Haddad Kashani H, Ehteram H, Banafshe H Clin Mol Allergy. 2022; 20(1):15.

PMID: 36550478 PMC: 9774079. DOI: 10.1186/s12948-022-00180-1.

Antibody tests for identification of current and past infection with SARS-CoV-2.

Fox T, Geppert J, Dinnes J, Scandrett K, Bigio J, Sulis G Cochrane Database Syst Rev. 2022; 11():CD013652.

PMID: 36394900 PMC: 9671206. DOI: 10.1002/14651858.CD013652.pub2.

Hospitalized children with SARS-CoV-2 infection and MIS-C in Jamaica: A dive into the first 15 months of the novel pandemic.

Berry C, Melbourne-Chambers R, Harrison A, Anzinger J, Gordon-Johnson K, Deyde V Front Pediatr. 2022; 10:904788.

PMID: 36160776 PMC: 9490120. DOI: 10.3389/fped.2022.904788.

References

Zhao J, Yuan Q, Wang H, Liu W, Liao X, Su Y . Antibody Responses to SARS-CoV-2 in Patients With Novel Coronavirus Disease 2019. Clin Infect Dis. 2020; 71(16):2027-2034. PMC: 7184337. DOI: 10.1093/cid/ciaa344. View

Wang C, Horby P, Hayden F, Gao G . A novel coronavirus outbreak of global health concern. Lancet. 2020; 395(10223):470-473. PMC: 7135038. DOI: 10.1016/S0140-6736(20)30185-9. View

Wang D, Hu B, Hu C, Zhu F, Liu X, Zhang J . Clinical Characteristics of 138 Hospitalized Patients With 2019 Novel Coronavirus-Infected Pneumonia in Wuhan, China. JAMA. 2020; 323(11):1061-1069. PMC: 7042881. DOI: 10.1001/jama.2020.1585. View

Liu R, Han H, Liu F, Lv Z, Wu K, Liu Y . Positive rate of RT-PCR detection of SARS-CoV-2 infection in 4880 cases from one hospital in Wuhan, China, from Jan to Feb 2020. Clin Chim Acta. 2020; 505:172-175. PMC: 7094385. DOI: 10.1016/j.cca.2020.03.009. View

Cai X, Chen J, Li Hu J, Long Q, Deng H, Liu P . A Peptide-Based Magnetic Chemiluminescence Enzyme Immunoassay for Serological Diagnosis of Coronavirus Disease 2019. J Infect Dis. 2020; 222(2):189-193. PMC: 7239108. DOI: 10.1093/infdis/jiaa243. View

Tate J, Ward G . Interferences in immunoassay. Clin Biochem Rev. 2008; 25(2):105-20. PMC: 1904417. View

Lou B, Li T, Zheng S, Su Y, Li Z, Liu W . Serology characteristics of SARS-CoV-2 infection after exposure and post-symptom onset. Eur Respir J. 2020; 56(2). PMC: 7401320. DOI: 10.1183/13993003.00763-2020. View

Kruttgen A, Cornelissen C, Dreher M, Hornef M, Imohl M, Kleines M . Comparison of four new commercial serologic assays for determination of SARS-CoV-2 IgG. J Clin Virol. 2020; 128:104394. PMC: 7189838. DOI: 10.1016/j.jcv.2020.104394. View

Roberton T, Carter E, Chou V, Stegmuller A, Jackson B, Tam Y . Early estimates of the indirect effects of the COVID-19 pandemic on maternal and child mortality in low-income and middle-income countries: a modelling study. Lancet Glob Health. 2020; 8(7):e901-e908. PMC: 7217645. DOI: 10.1016/S2214-109X(20)30229-1. View

10.

Ai T, Yang Z, Hou H, Zhan C, Chen C, Lv W . Correlation of Chest CT and RT-PCR Testing for Coronavirus Disease 2019 (COVID-19) in China: A Report of 1014 Cases. Radiology. 2020; 296(2):E32-E40. PMC: 7233399. DOI: 10.1148/radiol.2020200642. View

11.

Wang W, Xu Y, Gao R, Lu R, Han K, Wu G . Detection of SARS-CoV-2 in Different Types of Clinical Specimens. JAMA. 2020; 323(18):1843-1844. PMC: 7066521. DOI: 10.1001/jama.2020.3786. View

12.

Xie X, Zhong Z, Zhao W, Zheng C, Wang F, Liu J . Chest CT for Typical Coronavirus Disease 2019 (COVID-19) Pneumonia: Relationship to Negative RT-PCR Testing. Radiology. 2020; 296(2):E41-E45. PMC: 7233363. DOI: 10.1148/radiol.2020200343. View

13.

Huang Y, Cheng W, Zhao N, Qu H, Tian J . CT screening for early diagnosis of SARS-CoV-2 infection. Lancet Infect Dis. 2020; 20(9):1010-1011. PMC: 7195153. DOI: 10.1016/S1473-3099(20)30241-3. View

14.

Zhu N, Zhang D, Wang W, Li X, Yang B, Song J . A Novel Coronavirus from Patients with Pneumonia in China, 2019. N Engl J Med. 2020; 382(8):727-733. PMC: 7092803. DOI: 10.1056/NEJMoa2001017. View

15.

GeurtsvanKessel C, Okba N, Igloi Z, Bogers S, Embregts C, Laksono B . An evaluation of COVID-19 serological assays informs future diagnostics and exposure assessment. Nat Commun. 2020; 11(1):3436. PMC: 7338506. DOI: 10.1038/s41467-020-17317-y. View

16.

El-Sherif A, Elbahrawy A, Aboelfotoh A, Abdelkarim M, Saied Mohammad A, Abdallah A . High false-negative rate of anti-HCV among Egyptian patients on regular hemodialysis. Hemodial Int. 2012; 16(3):420-7. DOI: 10.1111/j.1542-4758.2011.00662.x. View