Esrrb Regulates Specific Feed-Forward Loops to Transit From Pluripotency Into Early Stages of Differentiation

Overview

Journal Front Cell Dev Biol

Specialty Cell Biology

Date 2022 Jun 2

PMID 35652095

Authors

Amin R Mazloom

Huilei Xu

Jaume Reig-Palou

Ana Vasileva

Angel-Carlos Roman

Sonia Mulero-Navarro

Ihor R Lemischka

Ana Sevilla

Affiliations

Soon will be listed here.

Abstract

Characterization of pluripotent states, in which cells can both self-renew or differentiate, with the irreversible loss of pluripotency, are important research areas in developmental biology. Although microRNAs (miRNAs) have been shown to play a relevant role in cellular differentiation, the role of miRNAs integrated into gene regulatory networks and its dynamic changes during these early stages of embryonic stem cell (ESC) differentiation remain elusive. Here we describe the dynamic transcriptional regulatory circuitry of stem cells that incorporate protein-coding and miRNA genes based on miRNA array expression and quantitative sequencing of short transcripts upon the downregulation of the Estrogen Related Receptor Beta (Esrrb). The data reveals how Esrrb, a key stem cell transcription factor, regulates a specific stem cell miRNA expression program and integrates dynamic changes of feed-forward loops contributing to the early stages of cell differentiation upon its downregulation. Together these findings provide new insights on the architecture of the combined transcriptional post-transcriptional regulatory network in embryonic stem cells.

Citing Articles

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PMID: 36078122 PMC: 9454435. DOI: 10.3390/cells11172714.

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