Conservation and Divergence of Myelin Proteome and Oligodendrocyte Transcriptome Profiles Between Humans and Mice

Overview

Journal Elife

Specialty Biology

Date 2022 May 11

PMID 35543322

Authors

Vasiliki-Ilya Gargareta

Josefine Reuschenbach

Sophie B Siems

Ting Sun

Lars Piepkorn

Carolina Mangana

Erik Spate

Sandra Goebbels

Inge Huitinga

Wiebke Mobius

Klaus-Armin Nave

Olaf Jahn

Hauke B Werner

Affiliations

Soon will be listed here.

Abstract

Human myelin disorders are commonly studied in mouse models. Since both clades evolutionarily diverged approximately 85 million years ago, it is critical to know to what extent the myelin protein composition has remained similar. Here, we use quantitative proteomics to analyze myelin purified from human white matter and find that the relative abundance of the structural myelin proteins PLP, MBP, CNP, and SEPTIN8 correlates well with that in C57Bl/6N mice. Conversely, multiple other proteins were identified exclusively or predominantly in human or mouse myelin. This is exemplified by peripheral myelin protein 2 (PMP2), which was specific to human central nervous system myelin, while tetraspanin-2 (TSPAN2) and connexin-29 (CX29/GJC3) were confined to mouse myelin. Assessing published scRNA-seq-datasets, human and mouse oligodendrocytes display well-correlating transcriptome profiles but divergent expression of distinct genes, including and . A searchable web interface is accessible via www.mpinat.mpg.de/myelin. Species-dependent diversity of oligodendroglial mRNA expression and myelin protein composition can be informative when translating from mouse models to humans.

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