The Chemopreventive Effects of Polyphenols and Coffee, Based Upon a DMBA Mouse Model with MicroRNA and MTOR Gene Expression Biomarkers

Overview

Journal Cells

Publisher MDPI

Specialties Biochemistry
Biophysics
Cell Biology
Molecular Biology

Date 2022 Apr 23

PMID 35455979

Authors

Richard Molnar

Laszlo Szabo

Andras Tomesz

Arpad Deutsch

Richard Darago

Bence L Raposa

Nowrasteh Ghodratollah

Timea Varjas

Balazs Nemeth

Zsuzsanna Orsos

Eva Pozsgai

Jozsef L Szentpeteri

Ferenc Budan

Istvan Kiss

Affiliations

Soon will be listed here.

Abstract

Polyphenols are capable of decreasing cancer risk. We examined the chemopreventive effects of a green tea () extract, polyphenol extract (a mixture of blackberry (), blackcurrants (), and added resveratrol phytoalexin), Chinese bayberry () extract, and a coffee () extract on 7,12-dimethylbenz[a]anthracene (DMBA) carcinogen-increased miR-134, miR-132, miR-124-1, miR-9-3, and gene expressions in the liver, spleen, and kidneys of CBA/Ca mice. The elevation was quenched significantly in the organs, except for miR-132 in the liver of the Chinese bayberry extract-consuming group, and miR-132 in the kidneys of the polyphenol-fed group. In the coffee extract-consuming group, only miR-9-3 and mTOR decreased significantly in the liver; also, miR-134 decreased significantly in the spleen, and, additionally, miR-124-1 decreased significantly in the kidney. Our results are supported by literature data, particularly the DMBA generated ROS-induced inflammatory and proliferative signal transducers, such as TNF, IL1, IL6, and NF-κB; as well as oncogenes, namely and . The examined chemopreventive agents, besides the obvious antioxidant and anti-inflammatory effects, mainly blocked the mentioned DMBA-activated factors and the mitogen-activated protein kinase (MAPK) as well, and, at the same time, induced as well as tumor suppressor genes.

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