Investigation of Toxoplasma Gondii in Wastewater and Surface Water in the Qinghai-Tibet Plateau, China Using Real-time PCR and Multilocus Genotyping

Overview

Journal Sci Rep

Specialty Science

Date 2022 Apr 1

PMID 35361820

Authors

Anna Lass

Ioannis Kontogeorgos

Liqing Ma

Xueyong Zhang

Xiuping Li

Panagiotis Karanis

Affiliations

Soon will be listed here.

Abstract

Toxoplasma gondii is a protozoan parasite, causing one of the most prevalent parasitic infections in the world. In the present study water sources of the Qinghai-Tibet Plateau (QTP), China, where the hygienic infrastructure is still developing, were investigated. A total of 214 water samples of 10 L volume, were collected from wastewater treatment plants (WWTPs), a slaughterhouse and rivers. The samples were filtered and then analysed using real-time PCR and multilocus genotyping. T. gondii DNA was found in four (1.9%) samples representing T. gondii type I; in one of them T. gondii-like oocysts were also confirmed microscopically. The approximate level of contamination of positive samples ranged between 30 and 2300 T. gondii sporozoites. The results of this study confirmed that T. gondii is present in wastewater in the greater metropolitan area of Xining and a neighbouring county. Contamination of wastewater at this level constitutes rather a moderate source of Toxoplasma infections in humans and animals. It suggests, however, a link between environmental exposure of animals, meat processing facilities and WWTPs. To our knowledge, this is the first investigation describing T. gondii detection in wastewater and environmental water samples collected from the territory of P.R. China using sensitive molecular tools.

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References

Ding H, Gao Y, Deng Y, Lamberton P, Lu D . A systematic review and meta-analysis of the seroprevalence of Toxoplasma gondii in cats in mainland China. Parasit Vectors. 2017; 10(1):27. PMC: 5237326. DOI: 10.1186/s13071-017-1970-6. View

Gallas-Lindemann C, Sotiriadou I, Mahmoodi M, Karanis P . Detection of Toxoplasma gondii oocysts in different water resources by Loop Mediated Isothermal Amplification (LAMP). Acta Trop. 2012; 125(2):231-6. DOI: 10.1016/j.actatropica.2012.10.007. View

Hatam-Nahavandi K, Mahvi A, Mohebali M, Keshavarz H, Mobedi I, Rezaeian M . Detection of parasitic particles in domestic and urban wastewaters and assessment of removal efficiency of treatment plants in Tehran, Iran. J Environ Health Sci Eng. 2015; 13:4. PMC: 4316801. DOI: 10.1186/s40201-015-0155-5. View

Gao Y, Ding H, Lamberton P, Lu D . Prevalence of Toxoplasma gondii in pet dogs in mainland China: A meta-analysis. Vet Parasitol. 2016; 229:126-130. DOI: 10.1016/j.vetpar.2016.10.009. View

Kourenti C, Karanis P . Evaluation and applicability of a purification method coupled with nested PCR for the detection of Toxoplasma oocysts in water. Lett Appl Microbiol. 2006; 43(5):475-81. DOI: 10.1111/j.1472-765X.2006.02008.x. View

Miller N, FRENKEL J, Dubey J . Oral infections with Toxoplasma cysts and oocysts in felines, other mammals, and in birds. J Parasitol. 1972; 58(5):928-37. View

Karanis P, Kimura A . Evaluation of three flocculation methods for the purification of Cryptosporidium parvum oocysts from water samples. Lett Appl Microbiol. 2002; 34(6):444-9. DOI: 10.1046/j.1472-765x.2002.01121.x. View

Wang M, Wang Y, Ye Q, Meng P, Yin H, Zhang D . Serological survey of Toxoplasma gondii in Tibetan mastiffs (Canis lupus familiaris) and yaks (Bos grunniens) in Qinghai, China. Parasit Vectors. 2012; 5:35. PMC: 3433318. DOI: 10.1186/1756-3305-5-35. View

Flegr J, Prandota J, Sovickova M, Israili Z . Toxoplasmosis--a global threat. Correlation of latent toxoplasmosis with specific disease burden in a set of 88 countries. PLoS One. 2014; 9(3):e90203. PMC: 3963851. DOI: 10.1371/journal.pone.0090203. View

10.

Tenter A, Heckeroth A, Weiss L . Toxoplasma gondii: from animals to humans. Int J Parasitol. 2000; 30(12-13):1217-58. PMC: 3109627. DOI: 10.1016/s0020-7519(00)00124-7. View

11.

Wells B, Shaw H, Innocent G, Guido S, Hotchkiss E, Parigi M . Molecular detection of Toxoplasma gondii in water samples from Scotland and a comparison between the 529bp real-time PCR and ITS1 nested PCR. Water Res. 2015; 87:175-81. DOI: 10.1016/j.watres.2015.09.015. View

12.

Cong W, Huang S, Zhou D, Zhang X, Zhang N, Zhao Q . Prevalence and genetic characterization of Toxoplasma gondii in house sparrows (Passer domesticus) in Lanzhou, China. Korean J Parasitol. 2013; 51(3):363-7. PMC: 3712113. DOI: 10.3347/kjp.2013.51.3.363. View

13.

Ma L, Sotiriadou I, Cai Q, Karanis G, Wang G, Wang G . Detection of Cryptosporidium and Giardia in agricultural and water environments in the Qinghai area of China by IFT and PCR. Parasitol Res. 2014; 113(9):3177-84. DOI: 10.1007/s00436-014-3979-5. View

14.

Shwab E, Zhu X, Majumdar D, Pena H, Gennari S, Dubey J . Geographical patterns of Toxoplasma gondii genetic diversity revealed by multilocus PCR-RFLP genotyping. Parasitology. 2014; 141(4):453-61. DOI: 10.1017/S0031182013001844. View

15.

DU F, Feng H, Nie H, Tu P, Zhang Q, Hu M . Survey on the contamination of Toxoplasma gondii oocysts in the soil of public parks of Wuhan, China. Vet Parasitol. 2011; 184(2-4):141-6. DOI: 10.1016/j.vetpar.2011.08.025. View

16.

Rajendran C, Su C, Dubey J . Molecular genotyping of Toxoplasma gondii from Central and South America revealed high diversity within and between populations. Infect Genet Evol. 2012; 12(2):359-68. DOI: 10.1016/j.meegid.2011.12.010. View

17.

Lass A, Szostakowska B, Korzeniewski K, Karanis P . The first detection of Toxoplasma gondii DNA in environmental air samples using gelatine filters, real-time PCR and loop-mediated isothermal (LAMP) assays: qualitative and quantitative analysis. Parasitology. 2017; 144(13):1791-1801. DOI: 10.1017/S0031182017001172. View

18.

Slany M, Dziedzinska R, Babak V, Kralik P, Moravkova M, Slana I . Toxoplasma gondii in vegetables from fields and farm storage facilities in the Czech Republic. FEMS Microbiol Lett. 2019; 366(14). DOI: 10.1093/femsle/fnz170. View

19.

Moura L, Bahia-Oliveira L, Wada M, Jones J, Tuboi S, Carmo E . Waterborne toxoplasmosis, Brazil, from field to gene. Emerg Infect Dis. 2006; 12(2):326-9. PMC: 3373086. DOI: 10.3201/eid1202.041115. View

20.

Galvani A, Christ A, Padula J, Barbosa M, de Araujo R, Sato M . Real-time PCR detection of Toxoplasma gondii in surface water samples in São Paulo, Brazil. Parasitol Res. 2019; 118(2):631-640. DOI: 10.1007/s00436-018-6185-z. View