Evaluation of Three Flocculation Methods for the Purification of Cryptosporidium Parvum Oocysts from Water Samples

Overview

Journal Lett Appl Microbiol

Publisher Oxford University Press

Specialty Microbiology

Date 2002 May 25

PMID 12028427

Citations 11

Authors

P Karanis

A Kimura

Affiliations

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Abstract

Aims: Evaluation of three flocculation methods for the purification of Cryptosporidium parvum oocysts from tap water.

Methods And Results: Ferric sulphate, aluminium sulphate and calcium carbonate were compared for their recovery efficiency of C. parvum oocysts from tap water. Lower mean recovery was achieved by calcium carbonate (38.8%) compared with ferric sulphate (61.5%) and aluminium sulphate (58.1%) for the recovery of 2.5 x 10(5) oocysts l(-1); 2.5 oocysts l(-1) and 1 oocyst l(-1) were adequately purified using ferric sulphate flocculation. In vitro excystation experiments showed that ferric sulphate flocculation does not markedly reduce the viability of oocysts.

Conclusions: Ferric sulphate flocculation is a simple and effective tool for the purification of C. parvum oocysts from tap water.

Significance And Impact Of The Study: The high recovery rates and low impact on oocyst viability provided by ferric sulphate flocculation might be useful for the detection of Cryptosporidium oocysts in environmental water samples.

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Lelis Giglio G, Sabogal-Paz L Environ Monit Assess. 2018; 190(11):686.

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