Molecular Identification of Protozoan in Different Types of Water Bodies in Lithuania

Overview

Journal Life (Basel)

Specialty Biology

Date 2023 Jan 21

PMID 36676000

Authors

Agne Baranauskaite

Zivile Strazdaite-Zieliene

Elena Serviene

Dalius Butkauskas

Petras Prakas

Affiliations

Soon will be listed here.

Abstract

Representatives of the genus are unicellular parasites having a two-host life cycle and infecting mammals, birds, and reptiles. Until now, spp. have been mainly investigated in definitive and intermediate hosts. Only a few studies have been conducted on the detection of parasites in water samples. The aim of this research was to examine whether the prevalence of spp. parasitizing farm animals varies in different types of water bodies. Water samples ( = 150) were collected from the entire territory of Lithuania, dividing water bodies into five groups (lakes, rivers, ponds/canals, swamps, and the inshore zone of the territorial Baltic Sea area). One-liter samples were filtered and subsequently analyzed using nested PCR. At least one of the analyzed spp. (. , . , . , . , . , . , . , and . ) was determined in all examined samples from water bodies. No significant difference in spp. prevalence between different types of water sources was detected. Our research proved that selecting appropriate primers is important for the accurate identification of parasites in samples collected from water bodies.

Citing Articles

Red foxes () and raccoon dogs () as potential spreaders of species.

Maca O, Gudiskis N, Butkauskas D, Gonzalez-Solis D, Prakas P Front Vet Sci. 2024; 11:1392618.

PMID: 38903682 PMC: 11188440. DOI: 10.3389/fvets.2024.1392618.

Detection of Three Species (Apicomplexa) in Blood Samples of the Bank Vole and Yellow-Necked Mouse from Lithuania.

Prakas P, Gudiskis N, Kitryte N, Bagdonaite D, Baltrunaite L Life (Basel). 2024; 14(3).

PMID: 38541690 PMC: 10971911. DOI: 10.3390/life14030365.

References

Dreelin E, Ives R, Molloy S, Rose J . Cryptosporidium and Giardia in surface water: a case study from Michigan, USA to inform management of rural water systems. Int J Environ Res Public Health. 2014; 11(10):10480-503. PMC: 4210991. DOI: 10.3390/ijerph111010480. View

Lass A, Kontogeorgos I, Ma L, Zhang X, Li X, Karanis P . Investigation of Toxoplasma gondii in wastewater and surface water in the Qinghai-Tibet Plateau, China using real-time PCR and multilocus genotyping. Sci Rep. 2022; 12(1):5428. PMC: 8971506. DOI: 10.1038/s41598-022-09166-0. View

Galvani A, Christ A, Padula J, Barbosa M, de Araujo R, Sato M . Real-time PCR detection of Toxoplasma gondii in surface water samples in São Paulo, Brazil. Parasitol Res. 2019; 118(2):631-640. DOI: 10.1007/s00436-018-6185-z. View

Gjerde B, Giacomelli S, Bianchi A, Bertoletti I, Mondani H, Gibelli L . Morphological and molecular characterization of four Sarcocystis spp., including Sarcocystis linearis n. sp., from roe deer (Capreolus capreolus) in Italy. Parasitol Res. 2017; 116(4):1317-1338. DOI: 10.1007/s00436-017-5410-5. View

Prakas P, Strazdaite-Zieliene Z, Januskevicius V, Chiesa F, Baranauskaite A, Rudaityte-Lukosiene E . Molecular identification of four Sarcocystis species in cattle from Lithuania, including S. hominis, and development of a rapid molecular detection method. Parasit Vectors. 2020; 13(1):610. PMC: 7720396. DOI: 10.1186/s13071-020-04473-9. View

Cabaj W, Grzelak S, Moskwa B, Bien-Kalinowska J . infection in free-living European bison ( L.) from the Białowieża Forest, Poland - A molecular analysis based on the gene. Int J Parasitol Parasites Wildl. 2021; 16:59-63. PMC: 8377485. DOI: 10.1016/j.ijppaw.2021.07.010. View

Dong H, Su R, Wang Y, Tong Z, Zhang L, Yang Y . Sarcocystis species in wild and domestic sheep (Ovis ammon and Ovis aries) from China. BMC Vet Res. 2018; 14(1):377. PMC: 6276146. DOI: 10.1186/s12917-018-1712-9. View

Reiling S, Merks H, Zhu S, Boone R, Corneau N, Dixon B . A cloth-based hybridization array system for rapid detection of the food- and waterborne protozoan parasites , spp. and . Food Waterborne Parasitol. 2021; 24:e00130. PMC: 8379661. DOI: 10.1016/j.fawpar.2021.e00130. View

Gjerde B, Luzon M, Alunda J, de la Fuente C . Morphological and molecular characteristics of six Sarcocystis spp. from red deer (Cervus elaphus) in Spain, including Sarcocystis cervicanis and three new species. Parasitol Res. 2017; 116(10):2795-2811. DOI: 10.1007/s00436-017-5590-z. View

10.

Alonso J, Amoros I, Guy R . Quantification of viable Giardia cysts and Cryptosporidium oocysts in wastewater using propidium monoazide quantitative real-time PCR. Parasitol Res. 2014; 113(7):2671-8. DOI: 10.1007/s00436-014-3922-9. View

11.

Lass A, Szostakowska B, Korzeniewski K, Karanis P . Detection of Giardia intestinalis in water samples collected from natural water reservoirs and wells in northern and north-eastern Poland using LAMP, real-time PCR and nested PCR. J Water Health. 2017; 15(5):775-787. DOI: 10.2166/wh.2017.039. View

12.

Caccio S, De Giacomo M, Aulicino F, Pozio E . Giardia cysts in wastewater treatment plants in Italy. Appl Environ Microbiol. 2003; 69(6):3393-8. PMC: 161540. DOI: 10.1128/AEM.69.6.3393-3398.2003. View

13.

Lee F . Finding Sarcocystis spp. on the Tioman Island: 28S rRNA gene next-generation sequencing reveals nine new Sarcocystis species. J Water Health. 2019; 17(3):416-427. DOI: 10.2166/wh.2019.124. View

14.

Strazdaite-Zieliene Z, Baranauskaite A, Butkauskas D, Serviene E, Prakas P . Molecular Identification of Parasitic Protozoa in Water Samples. Vet Sci. 2022; 9(8). PMC: 9412564. DOI: 10.3390/vetsci9080412. View

15.

Marandykina-Prakiene A, Butkauskas D, Gudiskis N, Juozaityte-Ngugu E, Januskevicius V, Rudaityte-Lukosiene E . Molecular Identification of Species in Sheep from Lithuania. Animals (Basel). 2022; 12(16). PMC: 9404443. DOI: 10.3390/ani12162048. View

16.

Karanis P, Kourenti C, Smith H . Waterborne transmission of protozoan parasites: a worldwide review of outbreaks and lessons learnt. J Water Health. 2007; 5(1):1-38. DOI: 10.2166/wh.2006.002. View

17.

Araoz V, Silveira C, More G, Banchero G, Riet-Correa F, Giannitti F . Fatal -induced eosinophilic myocarditis in a heifer in Uruguay. J Vet Diagn Invest. 2019; 31(4):656-660. PMC: 6857021. DOI: 10.1177/1040638719856651. View

18.

Nimri L . Unusual case presentation of intestinal infection in a healthy adult. JMM Case Rep. 2017; 1(4):e004069. PMC: 5415925. DOI: 10.1099/jmmcr.0.T00019. View

19.

Gallas-Lindemann C, Sotiriadou I, Mahmoodi M, Karanis P . Detection of Toxoplasma gondii oocysts in different water resources by Loop Mediated Isothermal Amplification (LAMP). Acta Trop. 2012; 125(2):231-6. DOI: 10.1016/j.actatropica.2012.10.007. View

20.

Prakas P, Kirillova V, Dzerkale A, Kirjusina M, Butkauskas D, Gavarane I . First molecular characterization of Sarcocystis miescheriana in wild boars (Sus scrofa) from Latvia. Parasitol Res. 2020; 119(11):3777-3783. DOI: 10.1007/s00436-020-06882-2. View