Can Wastewater Surveillance Enhance Genomic Tracking of Climate-Driven Pathogens?

Overview

Journal Microorganisms

Specialty Microbiology

Date 2025 Feb 26

PMID 40005661

Authors

Laura A E Van Poelvoorde

Erik A Karlsson

Myrielle Dupont-Rouzeyrol

Nancy H C J Roosens

Affiliations

Soon will be listed here.

Abstract

Climate change heightens the threat of infectious diseases in Europe, necessitating innovative surveillance methods. Based on 390 scientific papers, for the first time, this review associates climate-related pathogens, data related to their presence in wastewater, and associated available genomic detection methods. This deep analysis reveals a wide range of pathogens that can be tracked through methods such as quantitative and digital PCR, as well as genomic pathogen enrichment in combination with sequencing and metagenomics. Nevertheless, significant gaps remain in the development of methods, particularly for vector-borne pathogens, and in their general harmonization relating to performance criteria. By offering an overview of recent advancements while identifying critical gaps, we advocate for collaborative research and validation to integrate detection techniques into surveillance frameworks. This will enhance public health resilience against emerging infectious diseases driven by climate change.

References

Zatta M, Brichler S, Vindrios W, Melica G, Gallien S . Autochthonous Dengue Outbreak, Paris Region, France, September-October 2023. Emerg Infect Dis. 2023; 29(12):2538-2540. PMC: 10683815. DOI: 10.3201/eid2912.231472. View

Singh N, Lapierre P, Quinlan T, Halse T, Wirth S, Dickinson M . Whole-Genome Single-Nucleotide Polymorphism (SNP) Analysis Applied Directly to Stool for Genotyping Shiga Toxin-Producing Escherichia coli: an Advanced Molecular Detection Method for Foodborne Disease Surveillance and Outbreak Tracking. J Clin Microbiol. 2019; 57(7). PMC: 6595464. DOI: 10.1128/JCM.00307-19. View

Cuenca-Arias P, Montano L, Villarreal J, Wiesner M . Molecular and phenotypic characterization of Salmonella Typhimurium monophasic variant (1,4,[5],12:i:-) from Colombian clinical isolates. Biomedica. 2020; 40(4):722-733. PMC: 7808771. DOI: 10.7705/biomedica.5417. View

Martinez Viedma M, Puri V, Oldfield L, Shabman R, Tan G, Pickett B . Optimization of qRT-PCR assay for zika virus detection in human serum and urine. Virus Res. 2019; 263:173-178. PMC: 6561482. DOI: 10.1016/j.virusres.2019.01.013. View

Teklehaimanot G, Coetzee M, Momba M . Faecal pollution loads in the wastewater effluents and receiving water bodies: a potential threat to the health of Sedibeng and Soshanguve communities, South Africa. Environ Sci Pollut Res Int. 2014; 21(16):9589-603. DOI: 10.1007/s11356-014-2980-y. View

Humaidi M, Tien W, Yap G, Chua C, Ng L . Non-Invasive Dengue Diagnostics-The Use of Saliva and Urine for Different Stages of the Illness. Diagnostics (Basel). 2021; 11(8). PMC: 8393275. DOI: 10.3390/diagnostics11081345. View

Valledor S, Valledor I, Gil-Rodriguez M, Seral C, Castillo J . Comparison of several Real-Time PCR Kits versus a Culture-dependent Algorithm to Identify Enteropathogens in Stool Samples. Sci Rep. 2020; 10(1):4301. PMC: 7062723. DOI: 10.1038/s41598-020-61202-z. View

Li Z, Guan H, Wang W, Gao H, Feng W, Li J . Development of a Rapid and Fully Automated Multiplex Real-Time PCR Assay for Identification and Differentiation of and on the BD MAX Platform. Front Cell Infect Microbiol. 2021; 11:639473. PMC: 7947656. DOI: 10.3389/fcimb.2021.639473. View

Simner P, Oethinger M, Stellrecht K, Pillai D, Yogev R, Leblond H . Multisite Evaluation of the BD Max Extended Enteric Bacterial Panel for Detection of Yersinia enterocolitica, Enterotoxigenic Escherichia coli, Vibrio, and Plesiomonas shigelloides from Stool Specimens. J Clin Microbiol. 2017; 55(11):3258-3266. PMC: 5654910. DOI: 10.1128/JCM.00911-17. View

10.

Shukla S, Mittal V, Karoli R, Singh P, Singh A . Leptospirosis in central & eastern Uttar Pradesh, an underreported disease: A prospective cross-sectional study. Indian J Med Res. 2022; 155(1):66-72. PMC: 9552366. DOI: 10.4103/ijmr.IJMR_1811_19. View

11.

Haines A, Patz J . Health effects of climate change. JAMA. 2004; 291(1):99-103. DOI: 10.1001/jama.291.1.99. View

12.

Montasser K, Osman H, Abozaid H, Khalil H, Hatem Amer W, Sabry A . Multiplex PCR: Aid to more-timely and directed therapeutic intervention for patients with infectious gastroenteritis. Medicine (Baltimore). 2022; 101(41):e31022. PMC: 9575839. DOI: 10.1097/MD.0000000000031022. View

13.

Ulloa-Stanojlovic F, Aguiar B, Jara L, Sato M, Guerrero J, Hachich E . Occurrence of Giardia intestinalis and Cryptosporidium sp. in wastewater samples from São Paulo State, Brazil, and Lima, Peru. Environ Sci Pollut Res Int. 2016; 23(21):22197-22205. DOI: 10.1007/s11356-016-7537-9. View

14.

Heydari F, Bonyadian M, Moshtaghi H, Sami M . Prevalence and antibiotic resistance profile of Shiga toxin-producing isolated from diarrheal samples. Iran J Microbiol. 2020; 12(4):289-295. PMC: 7502143. DOI: 10.18502/ijm.v12i4.3931. View

15.

Iaconelli M, Purpari G, Della Libera S, Petricca S, Guercio A, Ciccaglione A . Hepatitis A and E Viruses in Wastewaters, in River Waters, and in Bivalve Molluscs in Italy. Food Environ Virol. 2015; 7(4):316-24. DOI: 10.1007/s12560-015-9207-3. View

16.

Porte L, Perez C, Barbe M, Varela C, Vollrath V, Legarraga P . spp. Prevalence in Santiago, Chile: A Study Based on Molecular Detection in Clinical Stool Samples from 2014 to 2019. Pathogens. 2023; 12(3). PMC: 10057968. DOI: 10.3390/pathogens12030504. View

17.

Mohapatra S, Samantaray J, Ghosh A . A Comparative Study of Serum, Urine and Saliva Using rk39 Strip for the Diagnosis of Visceral Leishmaniasis. J Arthropod Borne Dis. 2016; 10(1):87-91. PMC: 4813396. View

18.

Villar L, de Paula V, Diniz-Mendes L, Guimaraes F, Ferreira F, Shubo T . Molecular detection of hepatitis A virus in urban sewage in Rio de Janeiro, Brazil. Lett Appl Microbiol. 2007; 45(2):168-73. DOI: 10.1111/j.1472-765X.2007.02164.x. View

19.

Selvarajan R, Sibanda T, Pandian J, Mearns K . Taxonomic and Functional Distribution of Bacterial Communities in Domestic and Hospital Wastewater System: Implications for Public and Environmental Health. Antibiotics (Basel). 2021; 10(9). PMC: 8470611. DOI: 10.3390/antibiotics10091059. View

20.

Brehm T, Dupke S, Hauk G, Fickenscher H, Rohde H, Berneking L . [Non-cholera Vibrio species - currently still rare but growing danger of infection in the North Sea and the Baltic Sea]. Internist (Berl). 2021; 62(8):876-886. PMC: 8283098. DOI: 10.1007/s00108-021-01086-x. View