Neonatal LTβR Signaling is Required for the Accumulation of Eosinophils in the Inflamed Adult Mesenteric Lymph Node

Overview

Journal Mucosal Immunol

Publisher Elsevier

Specialty Allergy & Immunology

Date 2022 Feb 19

PMID 35181738

Authors

Conglei Li

Lesley A Ward

Albert Nguyen

Evelyn Lam

Dragos Dasoveanu

Musaddeque Ahmed

Kei Haniuda

Matthew B Buechler

Housheng Hansen He

Burkhard Ludewig

Kelly Marshall McNagny

Jennifer L Gommerman

Affiliations

Soon will be listed here.

Abstract

Although eosinophils are important contributors to mucosal immune responses, mechanisms that regulate their accumulation in mucosal-associated lymphoid tissues remain ill-defined. Combining bone marrow chimeras and pharmacological inhibition approaches, here we find that lymphotoxin-beta receptor (LTβR) signaling during the neonatal period is required for the accumulation of eosinophils in the mesenteric lymph nodes (MLN) during an enteric viral infection in adult male and female mice. We demonstrate that MLN stromal cells express genes that are important for eosinophil migration and survival, such as Ccl-11 (eotaxin-1), Ccl7, Ccl9, and Cxcl2, and that expression of most of these genes is downregulated as a consequence of neonatal LTβR blockade. We also find that neonatal LTβR signaling is required for the generation of a rotavirus-specific IgA antibody response in the adult MLN, but eosinophils are dispensable for this response. Collectively, our studies reveal a role for neonatal LTβR signaling in regulating eosinophil numbers in the adult MLN.

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