CRISPR-Cas-Led Revolution in Diagnosis and Management of Emerging Plant Viruses: New Avenues Toward Food and Nutritional Security

Overview

Journal Front Nutr

Specialty Nutritional Sciences

Date 2022 Jan 3

PMID 34977113

Citations 9

Authors

Susheel Kumar Sharma

Om Prakash Gupta

Neeta Pathaw

Devender Sharma

Albert Maibam

Parul Sharma

Jyotsana Sanasam

Suhas Gorakh Karkute

Sandeep Kumar

Bijoya Bhattacharjee

Affiliations

Soon will be listed here.

Abstract

Plant viruses pose a serious threat to agricultural production systems worldwide. The world's population is expected to reach the 10-billion mark by 2057. Under the scenario of declining cultivable land and challenges posed by rapidly emerging and re-emerging plant pathogens, conventional strategies could not accomplish the target of keeping pace with increasing global food demand. Gene-editing techniques have recently come up as promising options to enable precise changes in genomes with greater efficiency to achieve the target of higher crop productivity. Of genome engineering tools, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) proteins have gained much popularity, owing to their simplicity, reproducibility, and applicability in a wide range of species. Also, the application of different Cas proteins, such as Cas12a, Cas13a, and Cas9 nucleases, has enabled the development of more robust strategies for the engineering of antiviral mechanisms in many plant species. Recent studies have revealed the use of various CRISPR-Cas systems to either directly target a viral gene or modify a host genome to develop viral resistance in plants. This review provides a comprehensive record of the use of the CRISPR-Cas system in the development of antiviral resistance in plants and discusses its applications in the overall enhancement of productivity and nutritional landscape of cultivated plant species. Furthermore, the utility of this technique for the detection of various plant viruses could enable affordable and precise in-field or on-site detection. The futuristic potential of CRISPR-Cas technologies and possible challenges with their use and application are highlighted. Finally, the future of CRISPR-Cas in sustainable management of viral diseases, and its practical utility and regulatory guidelines in different parts of the globe are discussed systematically.

Citing Articles

Rapid and accurate detection of f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method.

Dai S, Wu Y, Zhu N, Zhao Y, Mao M, Li Z Front Plant Sci. 2025; 15:1485884.

PMID: 39759228 PMC: 11695371. DOI: 10.3389/fpls.2024.1485884.

Development of a CRISPR/SHERLOCK-Based Method for Rapid and Sensitive Detection of Selected Pospiviroids.

Zhai Y, Gnanasekaran P, Pappu H Viruses. 2024; 16(7).

PMID: 39066241 PMC: 11281484. DOI: 10.3390/v16071079.

Advances in RNA Interference for Plant Functional Genomics: Unveiling Traits, Mechanisms, and Future Directions.

Chaudhary D, Jeena A, Rohit , Gaur S, Raj R, Mishra S Appl Biochem Biotechnol. 2024; 196(9):5681-5710.

PMID: 38175411 DOI: 10.1007/s12010-023-04850-x.

CRISPR-Based Genome Editing Tools: An Accelerator in Crop Breeding for a Changing Future.

Zhang F, Neik T, Thomas W, Batley J Int J Mol Sci. 2023; 24(10).

PMID: 37239967 PMC: 10218198. DOI: 10.3390/ijms24108623.

Current and emerging trends in techniques for plant pathogen detection.

Venbrux M, Crauwels S, Rediers H Front Plant Sci. 2023; 14:1120968.

PMID: 37223788 PMC: 10200959. DOI: 10.3389/fpls.2023.1120968.

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